FCM/FACS (Flow Cytometry)
(CPN1 Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human CPN1 Polyclonal Antibody | anti-CPN1 antibody
CPN1 Antibody (Center)
Gene Names
CPN1; CPN; SCPN
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
CPN1, Antibody; CPN1 Antibody (Center); Carboxypeptidase N catalytic chain; CPN; Anaphylatoxin inactivator; Arginine carboxypeptidase; Carboxypeptidase N polypeptide 1; Carboxypeptidase N small subunit; Kininase-1; Lysine carboxypeptidase; Plasma carboxypeptidase B; Serum carboxypeptidase N; SCPN; CPN1; ACBP; anti-CPN1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This CPN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 210-240 amino acids from the Central region of human CPN1.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Sequence Positions
210-240
Sequence Length
458
Applicable Applications for anti-CPN1 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Function
Protects the body from potent vasoactive and inflammatory peptides containing C-terminal Arg or Lys (such as kinins or anaphylatoxins) which are released into the circulation.
Cellular Location
Secreted, extracellular space.
Tissue Location
Synthesized in the liver and secreted in plasma.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(CPN1 Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(CPN1 Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(CPN1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CPN1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(CPN1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CPN1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of CPN1 (arrow) using rabbit polyclonal CPN1 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CPN1 gene.)
WB (Western Blot)
(Western blot analysis of CPN1 (arrow) using rabbit polyclonal CPN1 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CPN1 gene.)
Related Product Information for anti-CPN1 antibody
Carboxypeptidase N is a plasma metallo-protease that
cleaves basic amino acids from the C terminal of peptides and
proteins. The enzyme is important in the regulation of peptides
like kinins and anaphylatoxins, and has also been known as
kininase-1 and anaphylatoxin inactivator. This enzyme is a tetramer
comprised of two identical regulatory subunits and two identical
catalytic subunits; this gene encodes the catalytic subunit.
Mutations in this gene can be associated with angioedema or chronic
urticaria resulting from carboxypeptidase N deficiency. [provided
by RefSeq].
cleaves basic amino acids from the C terminal of peptides and
proteins. The enzyme is important in the regulation of peptides
like kinins and anaphylatoxins, and has also been known as
kininase-1 and anaphylatoxin inactivator. This enzyme is a tetramer
comprised of two identical regulatory subunits and two identical
catalytic subunits; this gene encodes the catalytic subunit.
Mutations in this gene can be associated with angioedema or chronic
urticaria resulting from carboxypeptidase N deficiency. [provided
by RefSeq].
Product Categories/Family for anti-CPN1 antibody
References
Speliotes, E.K., et al. Hepatology 52(3):904-912(2010)
Ichikawa, S., et al. J. Bone Miner. Res. 25(8):1821-1829(2010)
Du, X.Y., et al. J. Biol. Chem. 284(2):751-758(2009)
Yuan, X., et al. Am. J. Hum. Genet. 83(4):520-528(2008)
Skidgel, R.A., et al. Int. Immunopharmacol. 7(14):1888-1899(2007)
Ichikawa, S., et al. J. Bone Miner. Res. 25(8):1821-1829(2010)
Du, X.Y., et al. J. Biol. Chem. 284(2):751-758(2009)
Yuan, X., et al. Am. J. Hum. Genet. 83(4):520-528(2008)
Skidgel, R.A., et al. Int. Immunopharmacol. 7(14):1888-1899(2007)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
carboxypeptidase N catalytic chain
NCBI Official Synonym Full Names
carboxypeptidase N, polypeptide 1
NCBI Official Symbol
CPN1
NCBI Official Synonym Symbols
CPN; SCPN
NCBI Protein Information
carboxypeptidase N catalytic chain
UniProt Protein Name
Carboxypeptidase N catalytic chain
UniProt Gene Name
CPN1
UniProt Synonym Gene Names
ACBP; CPN; SCPN
UniProt Entry Name
CBPN_HUMAN
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Product Notes
The CPN1 cpn1 (Catalog #AAA284453) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 210-240. The CPN1 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CPN1 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the CPN1 cpn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CPN1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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