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IHC (Immunohistochemistry)
(Anti-Cpn10 antibody, AAA45158, IHC(P)IHC(P): Rat Intestine Tissue)
Rabbit Cpn10 Polyclonal Antibody | anti-HSPE1 antibody
Anti-Cpn10 antibody
Gene Names
HSPE1; EPF; CPN10; GROES; HSP10
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Synonyms
Cpn10, Antibody; Anti-Cpn10 antibody; 10 kDa heat shock protein, mitochondrial(Hsp10); heat shock 10kDa protein 1(chaperonin 10); 10 kDa chaperonin antibody; 10 kDa heat shock protein mitochondrial antibody; 10 kDa heat shock protein, mitochondrial antibody; CH10_HUMAN antibody; Chaperonin 10 antibody; Cpn 10 antibody; CPN10 antibody; Early pregnancy factor antibody; Early-pregnancy factor antibody; EPF antibody; GROES antibody; Heat shock 10kD protein 1 antibody; Heat shock 10kD protein 1 chaperonin 10 antibody; Heat shock 10kDa protein 1 antibody; Heat shock 10kDa protein 1 chaperonin 10 antibody; HSP 10 antibody; HSP10 antibody; HSPE 1 antibody; HSPE1 antibody; anti-HSPE1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
102
Applicable Applications for anti-HSPE1 antibody
ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Cpn10 (81-102aa VVLDDKDYFLFRDGDILGKYVD), different from the related rat and mouse sequences by one amino acid.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
IHC (Immunohistochemistry)
(Anti-Cpn10 antibody, AAA45158, IHC(P)IHC(P): Rat Intestine Tissue)
IHC (Immunohistochemistry)
(Anti-Cpn10 antibody, AAA45158, IHC(P)IHC(P): Rat Intestine Tissue)
IHC (Immunohistochemistry)
(Anti-Cpn10 antibody, AAA45158, IHC(P)IHC(P): Mouse Intestine Tissue)
IHC (Immunohistochemistry)
(Anti-Cpn10 antibody, AAA45158, IHC(P)IHC(P): Mouse Intestine Tissue)
IHC (Immunohistochemisry)
(Anti-Cpn10 antibody, AAA45158, IHC(P)IHC(P): Human Intestinal Cancer Tissue)
IHC (Immunohistochemisry)
(Anti-Cpn10 antibody, AAA45158, IHC(P)IHC(P): Human Intestinal Cancer Tissue)
WB (Western Blot)
(Anti-Cpn10 antibody, AAA45158, Western blottingAll lanes: Anti Cpn10 (AAA45158) at 0.5ug/mlLane 1: Rat Thymus Tissue Lysate at 50ugLane 2: Rat Brain Tissue Lysate at 50ugLane 3: Rat Ovary Tissue Lysate at 50ugLane 4: Rat Testis Tissue Lysate at 50ugLane 5: A431 Whole Cell Lysate at 40ugLane 6: A549 Whole Cell Lysate at 40ugLane 7: MCF-7 Whole Cell Lysate at 40ugLane 8: MM231 Whole Cell Lysate at 40ugLane 9: HELA Whole Cell Lysate at 40ugPredicted bind size: 11KDObserved bind size: 11KD)
WB (Western Blot)
(Anti-Cpn10 antibody, AAA45158, Western blottingAll lanes: Anti Cpn10 (AAA45158) at 0.5ug/mlLane 1: Rat Thymus Tissue Lysate at 50ugLane 2: Rat Brain Tissue Lysate at 50ugLane 3: Rat Ovary Tissue Lysate at 50ugLane 4: Rat Testis Tissue Lysate at 50ugLane 5: A431 Whole Cell Lysate at 40ugLane 6: A549 Whole Cell Lysate at 40ugLane 7: MCF-7 Whole Cell Lysate at 40ugLane 8: MM231 Whole Cell Lysate at 40ugLane 9: HELA Whole Cell Lysate at 40ugPredicted bind size: 11KDObserved bind size: 11KD)
ICC (Immunocytochemistry)
(Anti-Cpn10 antibody, AAA45158, ICCICC: A549 Cell)
ICC (Immunocytochemistry)
(Anti-Cpn10 antibody, AAA45158, ICCICC: A549 Cell)
Related Product Information for anti-HSPE1 antibody
Description: Rabbit IgG polyclonal antibody for 10 kDa heat shock protein, mitochondrial(HSPE1) detection. Tested with WB, IHC-P, ICC in Human, Mouse, Rat.
Background: HSPE1(heat shock 10kDa protein 1(chaperonin 10)), also called CPN10, GROES, CHAPERONIN 10 HOMOLOG, cpn10 HOMOLOG or HSP10, is a protein that in humans is encoded by the HSPE1 gene. GroES is a heptameric ring of identical 10.4-kD subunits that binds to each end of GroEL to form a symmetric, functional heterodimer. The HSP10 gene consists of 4 exons. The HSPE1 gene is mapped to 2q33.1. The transcriptional activity of the promoter fragment in the HSP60 direction is approximately twice that in the HSP10 direction under normal growth conditions; upon heat shock, promoter activity in either direction increased by a factor of approximately 12. Mutational drifts performed in vitro with 4 different enzymes indicated the GroES overexpression doubled the number of accumulating mutations, and promoted the folding of enzyme variants carrying mutations in the protein core and/or mutations with higher destabilizing effects.
Background: HSPE1(heat shock 10kDa protein 1(chaperonin 10)), also called CPN10, GROES, CHAPERONIN 10 HOMOLOG, cpn10 HOMOLOG or HSP10, is a protein that in humans is encoded by the HSPE1 gene. GroES is a heptameric ring of identical 10.4-kD subunits that binds to each end of GroEL to form a symmetric, functional heterodimer. The HSP10 gene consists of 4 exons. The HSPE1 gene is mapped to 2q33.1. The transcriptional activity of the promoter fragment in the HSP60 direction is approximately twice that in the HSP10 direction under normal growth conditions; upon heat shock, promoter activity in either direction increased by a factor of approximately 12. Mutational drifts performed in vitro with 4 different enzymes indicated the GroES overexpression doubled the number of accumulating mutations, and promoted the folding of enzyme variants carrying mutations in the protein core and/or mutations with higher destabilizing effects.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
10,932 Da
NCBI Official Full Name
10 kDa heat shock protein, mitochondrial
NCBI Official Synonym Full Names
heat shock 10kDa protein 1
NCBI Official Symbol
HSPE1
NCBI Official Synonym Symbols
EPF; CPN10; GROES; HSP10
NCBI Protein Information
10 kDa heat shock protein, mitochondrial; chaperonin 10; 10 kDa chaperonin; early-pregnancy factor; heat shock 10kD protein 1 (chaperonin 10)
UniProt Protein Name
10 kDa heat shock protein, mitochondrial
UniProt Gene Name
HSPE1
UniProt Synonym Gene Names
Hsp10; CPN10; EPF
UniProt Entry Name
CH10_HUMAN
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Product Notes
The HSPE1 hspe1 (Catalog #AAA45158) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Cpn10 antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Cpn10 can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the HSPE1 hspe1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cpn10, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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