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product-image-AAA162774_IHC10.jpg IHC (Immunohistochemistry) (CREBBP/CREB Binding Protein Antibody-Immunohistochemical analysis of CBP staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.)

Rabbit CREBBP/CREB Binding Protein Polyclonal Antibody | anti-CREBBP antibody

CREBBP/CREB Binding Protein Rabbit anti-Human Polyclonal (C-Terminus) Antibody

Gene Names
CREBBP; CBP; RSTS; KAT3A
Reactivity
Chicken, Bovine, Mouse, Dog, Rat, Pig, Human
Applications
Western Blot, Immunohistochemistry, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
Immunoaffinity purified
Synonyms
CREBBP/CREB Binding Protein, Antibody; CREBBP/CREB Binding Protein Rabbit anti-Human Polyclonal (C-Terminus) Antibody; IHC-plus CREBBP/CREB Binding Protein Antibody (C-Terminus); CREBBP; CREB binding protein; CREB-binding protein; KAT3A; RSTS; Rubinstein-Taybi syndrome; RTS; CBP; anti-CREBBP antibody
Ordering
Host
Rabbit
Reactivity
Chicken, Bovine, Mouse, Dog, Rat, Pig, Human
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of CBP protein.
Purity/Purification
Immunoaffinity purified
Form/Format
PBS, pH7.3, 0.01% Sodium Azide, 30% Glycerol
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-CREBBP antibody
WB (Western Blot), IHC (Immunohistochemistry), IHC (Immunohistochemistry), IF (Immunofluorescence), ICC (Immunocytochemistry)
Target
Human CREBBP/CREB Binding Protein
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-Terminus of human CBP.
Conjugation
Unconjugated
Epitope
C-Terminus
Preparation and Storage
Store at -20 degree C. Aliquot to avoid freeze/thaw cycles.

IHC (Immunohistochemistry)

(CREBBP/CREB Binding Protein Antibody-Immunohistochemical analysis of CBP staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.)

product-image-AAA162774_IHC10.jpg IHC (Immunohistochemistry) (CREBBP/CREB Binding Protein Antibody-Immunohistochemical analysis of CBP staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.)

IHC (Immunohistochemisry)

(CREBBP/CREB Binding Protein Antibody-Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE))

product-image-AAA162774_IHC11.jpg IHC (Immunohistochemisry) (CREBBP/CREB Binding Protein Antibody-Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE))

WB (Western Blot)

(CREBBP/CREB Binding Protein Antibody-Western blot analysis of CBP expression in HeLa (A); A431 (B); HT29 (C); A549 (D) whole cell lysates.)

product-image-AAA162774_WB13.jpg WB (Western Blot) (CREBBP/CREB Binding Protein Antibody-Western blot analysis of CBP expression in HeLa (A); A431 (B); HT29 (C); A549 (D) whole cell lysates.)

ICC (Immunocytochemistry)

(CREBBP/CREB Binding Protein Antibody-Immunofluorescent analysis of CBP staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)

product-image-AAA162774_ICC15.jpg ICC (Immunocytochemistry) (CREBBP/CREB Binding Protein Antibody-Immunofluorescent analysis of CBP staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Related Product Information for anti-CREBBP antibody
CREB Binding Protein antibody is an unconjugated rabbit polyclonal antibody to CREB Binding Protein (CREBBP)(C-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
260,993 Da
NCBI Official Full Name
CREB-binding protein isoform a
NCBI Official Synonym Full Names
CREB binding protein
NCBI Official Symbol
CREBBP
NCBI Official Synonym Symbols
CBP; RSTS; KAT3A
NCBI Protein Information
CREB-binding protein
UniProt Protein Name
CREB-binding protein
UniProt Gene Name
CREBBP
UniProt Synonym Gene Names
CBP
UniProt Entry Name
CBP_HUMAN

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Product Notes

The CREBBP crebbp (Catalog #AAA162774) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CREBBP/CREB Binding Protein Rabbit anti-Human Polyclonal (C-Terminus) Antibody reacts with Chicken, Bovine, Mouse, Dog, Rat, Pig, Human and may cross-react with other species as described in the data sheet. AAA Biotech's CREBBP/CREB Binding Protein can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), IHC (Immunohistochemistry), IF (Immunofluorescence), ICC (Immunocytochemistry). Researchers should empirically determine the suitability of the CREBBP crebbp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CREBBP/CREB Binding Protein, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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