Rabbit anti-Human CRELD1 Polyclonal Antibody | anti-CRELD1 antibody

Anti-CRELD1 Antibody, Rabbit Polyclonal

Cat. Num. AAA258909

• Reactivity: Human
• Applications: Immunoprecipitation, Western Blot, ELISA
• Purity: Protein A & Antigen Affinity
• Synonyms: CRELD1, Antibody; Anti-CRELD1 Antibody, Rabbit Polyclonal; cysteine-rich with EGF-like domains 1; Anti-AVSD2 Antibody; Anti-CIRRIN Antibody; anti-CRELD1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human CRELD1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CRELD1 antibody: IP (Immunoprecipitation), WB (Western Blot), ELISA
• Immunogen: Recombinant Human CRELD1 Protein
• Preparation: Produced in rabbits immunized with purified, recombinant Human CRELD1 (rh CRELD1). CRELD1 specific IgG was purified by Human CRELD1 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

IP (Immunoprecipitation)

(CRELD1 was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell Lysate4 uL anti-CRELD1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-CRELD1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 46 kDaObserved band size :46 kDa)

WB (Western Blot)

(Anti-CRELD1 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:46 kDaObserved band size:46 kDa)

Related Product Information for anti-CRELD1 antibody

CRELD1 is a transmembrane glycoprotein. Epidermal growth factor(EGF)-like domain exists in CRELD1. EGF-like repeats are a class of cysteine-rich domains that mediate interactions between proteins of diverse function. EGF domains are found in proteins that are either completely secreted or have transmembrane regions that tether the protein to the cell surface. CRELD1 contains a 333 amino acid acid (aa) extracellular domain (ECD), two tandem transmembrane segments, and a second ECD of 15 aa. Defects in CRELD1 may cause susceptibility to atrioventricular septal defect type 2 which results in a persistent common atrioventricular canal.

References

Robinson SW, et al. (2003) Missense Mutations in CRELD1 Are Associated with Cardiac Atrioventricular Septal Defects. Am J Hum Genet. 72(4):1047-52.
Zatyka M, et al. (2005) Analysis of CRELD1 as a candidate 3p25 atrioventicular septal defect locus (AVSD2). Clin Genet. 67(6):526-8.
Stelzl U, et al. (2005) A human protein-protein interaction network: a resource for annotating the proteome. Cell. 122(6):957-68.