Rabbit CRFR1 Polyclonal Antibody | anti-CRHR1 antibody

CRFR1 Antibody (Q103)

Cat. Num. AAA283659

• Gene Names: CRHR1; CRF1; CRHR; CRF-R; CRFR1; CRF-R1; CRFR-1; CRH-R1; CRHR1L; CRF-R-1; CRH-R-1
• Reactivity: Human (Predicted Reactivity: Rat)
• Applications: Flow Cytometry, Functional Assay, ELISA, Western Blot
• Purity: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Synonyms: CRFR1, Antibody; CRFR1 Antibody (Q103); Corticotropin-releasing factor receptor 1; CRF-R-1; CRF-R1; CRFR-1; Corticotropin-releasing hormone receptor 1; CRH-R-1; CRH-R1; CRHR1; CRFR; CRFR1; CRHR; anti-CRHR1 antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Rat)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: This CRFR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 88-119 amino acids from human CRFR1.
• Purity/Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Positions: 88-119
• Sequence Length: 444

• Applicable Applications for anti-CRHR1 antibody: FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
• Function: G-protein coupled receptor for CRH (corticotropin-releasing factor) and UCN (urocortin). Has high affinity for CRH and UCN. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and down-stream effectors, such as adenylate cyclase. Promotes the activation of adenylate cyclase, leading to increased intracellular cAMP levels. Inhibits the activity of the calcium channel CACNA1H. Required for normal embryonic development of the adrenal gland and for normal hormonal responses to stress. Plays a role in the response to anxiogenic stimuli.
• Cellular Location: Cell membrane; Multi-pass membrane protein. Endosome. Note=Agonist-binding promotes endocytosis
• Tissue Location: Predominantly expressed in the cerebellum, pituitary, cerebral cortex and olfactory lobe
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

FCM/FACS (Flow Cytometry)

(CRFR1 Antibody (Q103) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

WB (Western Blot)

(CRFR1 Antibody (Q103) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the CRFR1 antibody detected the CRFR1 protein (arrow).)

Related Product Information for anti-CRHR1 antibody

This gene encodes a G-protein coupled receptor that binds
neuropeptides of the corticotropin releasing hormone family that
are major regulators of the hypothalamic-pituitary-adrenal pathway.
The encoded protein is essential for the activation of signal
transduction pathways that regulate diverse physiological processes
including stress, reproduction, immune response and obesity.
Alternative splicing results in multiple transcript variants.

Product Categories/Family for anti-CRHR1 antibody

Metabolism; Neuroscience; Signal Transduction; GPCR Antibodies

References

Karteris, E., et al. Endocrinology 151(10):4959-4968(2010)
Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010) :
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Ruano, G., et al. Pharmacogenomics 11(7):959-971(2010)
Hillhouse, E.W., et al. Endocr. Rev. 27(3):260-286(2006)

NCBI and Uniprot Product Information

• NCBI GI #: 223717971
• NCBI GeneID: 1394
• NCBI Accession #: NP_001138618.1; NP_001138619.1; NP_001138620.1; NP_001243228.1; NP_004373.2
• NCBI GenBank Nucleotide #: NM_001145146.1
• UniProt Accession #: P34998; Q13008; Q4QRJ1; Q9UK64; B4DIE9
• Molecular Weight: 47671
• NCBI Official Full Name: corticotropin-releasing factor receptor 1 isoform 1
• NCBI Official Synonym Full Names: corticotropin releasing hormone receptor 1
• NCBI Official Symbol: CRHR1
• NCBI Official Synonym Symbols: CRF1; CRHR; CRF-R; CRFR1; CRF-R1; CRFR-1; CRH-R1; CRHR1L; CRF-R-1; CRH-R-1
• NCBI Protein Information: corticotropin-releasing factor receptor 1
• UniProt Protein Name: Corticotropin-releasing factor receptor 1
• UniProt Gene Name: CRHR1
• UniProt Synonym Gene Names: CRFR; CRFR1; CRHR; CRF-R-1; CRF-R1; CRFR-1; CRH-R-1; CRH-R1
• UniProt Entry Name: CRFR1_HUMAN