FCM/FACS (Flow Cytometry)
(CRFR2D Antibody flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human CRFR2D Polyclonal Antibody | anti-CRHR2 antibody
CRFR2D Antibody
Gene Names
CRHR2; CRF2; CRFR2; CRF-RB; HM-CRF
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
CRFR2D, Antibody; CRFR2D Antibody; Corticotropin-releasing factor receptor 2; CRF-R-2; CRF-R2; CRFR-2; Corticotropin-releasing hormone receptor 2; CRH-R-2; CRH-R2; CRHR2; CRF2R; CRH2R; anti-CRHR2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This CRFR2D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the human CRFR2D.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Length
410
Applicable Applications for anti-CRHR2 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Cellular Location
Cell membrane; Multi-pass membrane protein
Antigen Source
HUMAN
Function
G-protein coupled receptor for CRH (corticotropin-releasing factor), UCN (urocortin), UCN2 and UCN3. Has high affinity for UCN. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and down-stream effectors, such as adenylate cyclase. Promotes the activation of adenylate cyclase, leading to increased intracellular cAMP levels.
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(CRFR2D Antibody flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(CRFR2D Antibody flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(CRFR2D Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CRFR2D Antibody for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(CRFR2D Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CRFR2D Antibody for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(CRFR2D Antibody western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the CRFR2D antibody detected the CRFR2D protein (arrow).)
WB (Western Blot)
(CRFR2D Antibody western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the CRFR2D antibody detected the CRFR2D protein (arrow).)
Related Product Information for anti-CRHR2 antibody
Liaw et al. (1996) [PubMed 8536644] stated that there are 2 G protein-coupled corticotropin-releasing hormone (CRH; MIM 122560) receptors, CRHR1 (MIM 122561) and CRHR2, which they termed the CRF2 receptor. CRH is a 41-amino acid peptide synthesized in the hypothalamus that is the principal neuroregulator of the hypothalamic-pituitary-adrenocortical axis and plays an important role in coordinating the endocrine, autonomic, and behavioral responses to stress and immune challenge.
Product Categories/Family for anti-CRHR2 antibody
References
Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010) :
Gutknecht, E., et al. Eur. J. Pharmacol. 642 (1-3), 1-9 (2010) :
Ruano, G., et al. Pharmacogenomics 11(7):959-971(2010)
Pinheiro, A.P., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 153B (5), 1070-1080 (2010) :
Joubert, B.R., et al. Genome Med 2 (3), 17 (2010) :
Gutknecht, E., et al. Eur. J. Pharmacol. 642 (1-3), 1-9 (2010) :
Ruano, G., et al. Pharmacogenomics 11(7):959-971(2010)
Pinheiro, A.P., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 153B (5), 1070-1080 (2010) :
Joubert, B.R., et al. Genome Med 2 (3), 17 (2010) :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
47688
NCBI Official Full Name
corticotropin-releasing factor receptor 2 isoform 2
NCBI Official Synonym Full Names
corticotropin releasing hormone receptor 2
NCBI Official Symbol
CRHR2
NCBI Official Synonym Symbols
CRF2; CRFR2; CRF-RB; HM-CRF
NCBI Protein Information
corticotropin-releasing factor receptor 2
UniProt Protein Name
Corticotropin-releasing factor receptor 2
UniProt Gene Name
CRHR2
UniProt Synonym Gene Names
CRF2R; CRH2R; CRF-R-2; CRF-R2; CRFR-2; CRH-R-2; CRH-R2
UniProt Entry Name
CRFR2_HUMAN
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Product Notes
The CRHR2 crhr2 (Catalog #AAA287419) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CRFR2D Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CRFR2D can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the CRHR2 crhr2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CRFR2D, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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