WB (Western Blot)
(Detection of human and mouse CrkRS by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CrkRS antibody AAA211586 (lot AAA211586-3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human CrkRS Polyclonal Antibody | anti-CrkRS antibody
Rabbit anti-CrkRS Antibody, Affinity Purified
Gene Names
CDK12; CRK7; CRKR; CRKRS
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
CrkRS, Antibody; Rabbit anti-CrkRS Antibody, Affinity Purified; cell division cycle 2-related protein kinase 7; hCDK12; cyclin-dependent kinase 12; CRKRS; CRKR; cell division protein kinase 12; CDC2-related protein kinase 7; Cdc2-related kinase, arginine/serine-rich; CRK7; Cyclin-dependent kinase 12; cyclin dependent kinase 12; anti-CrkRS antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-CrkRS antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Mouse, Rat
Immunogen
Between 1 and 50
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse CrkRS by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CrkRS antibody AAA211586 (lot AAA211586-3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human and mouse CrkRS by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CrkRS antibody AAA211586 (lot AAA211586-3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human CrkRS by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CrkRS antibody AAA211586 (lot AAA211586-3) used for IP at 6 ug per reaction. For blotting immunoprecipitated CrkRS, AAA211586 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human CrkRS by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CrkRS antibody AAA211586 (lot AAA211586-3) used for IP at 6 ug per reaction. For blotting immunoprecipitated CrkRS, AAA211586 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Related Product Information for anti-CrkRS antibody
CrkRS (Cdc2-related kinase, arginine/serine-rich) is a Cdc-2 related kinase that contains an arginine- and serine-rich (SR) domain and has recently been reported to interact with cyclin L1 and L2. Since it has been discovered that CrkRs pairs with a cyclin partner, it has also been given the name CDK12. CrkRs localizes to nuclear speckles and immunoprecipitates with RNA polymerse II and the SF2/ASF splicing factor and is proposed to play a role in the regulation of alternative splicing.
Product Categories/Family for anti-CrkRS antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
141,449 Da
NCBI Official Full Name
cyclin-dependent kinase 12 isoform 1
NCBI Official Synonym Full Names
cyclin-dependent kinase 12
NCBI Official Symbol
CDK12
NCBI Official Synonym Symbols
CRK7; CRKR; CRKRS
NCBI Protein Information
cyclin-dependent kinase 12; CDC2-related protein kinase 7; cell division protein kinase 12; Cdc2-related kinase, arginine/serine-rich; cell division cycle 2-related protein kinase 7
UniProt Protein Name
Cyclin-dependent kinase 12
UniProt Gene Name
CDK12
UniProt Synonym Gene Names
CRK7; CRKRS; KIAA0904; CrkRS; CDC2-related protein kinase 7; hCDK12
UniProt Entry Name
CDK12_HUMAN
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Product Notes
The CrkRS cdk12 (Catalog #AAA211586) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-CrkRS Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CrkRS can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the CrkRS cdk12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CrkRS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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