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product-image-AAA289096_WB11.jpg WB (Western Blot) (CRTAP Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the CRTAP antibody detected the CRTAP protein (arrow).)

Rabbit anti-Human CRTAP Polyclonal Antibody | anti-CRTAP antibody

CRTAP Antibody (N-term)

Gene Names
CRTAP; OI7; CASP; P3H5; LEPREL3
Reactivity
Human
Applications
Western Blot, ELISA, Immunofluorescence
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
CRTAP, Antibody; CRTAP Antibody (N-term); Cartilage-associated protein; CRTAP; CASP; anti-CRTAP antibody
Ordering
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This CRTAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 31-59 amino acids from the N-terminal region of human CRTAP.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
31-59
Sequence Length
401
Applicable Applications for anti-CRTAP antibody
WB (Western Blot), ELISA, IF (Immunofluorescence)
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(CRTAP Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the CRTAP antibody detected the CRTAP protein (arrow).)

product-image-AAA289096_WB11.jpg WB (Western Blot) (CRTAP Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the CRTAP antibody detected the CRTAP protein (arrow).)

WB (Western Blot)

(Western blot analysis of lysates from HT-1080, ZR-75-1 cell line (from left to right), using CRTAP Antibody (N-term). AAA289096 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)

product-image-AAA289096_WB13.jpg WB (Western Blot) (Western blot analysis of lysates from HT-1080, ZR-75-1 cell line (from left to right), using CRTAP Antibody (N-term). AAA289096 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)

IF (Immunofluorescence)

(Fluorescent image of Hela cells stained with CRTAP Antibody (N-term). AAA289096 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)

product-image-AAA289096_IF15.jpg IF (Immunofluorescence) (Fluorescent image of Hela cells stained with CRTAP Antibody (N-term). AAA289096 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
Related Product Information for anti-CRTAP antibody
The protein encoded by this gene is similar to the chicken
and mouse CRTAP genes. The encoded protein is a scaffolding protein
that may influence the activity of at least one member of the
cytohesin/ARNO family in response to specific cellular stimuli.
Defects in this gene are associated with osteogenesis imperfecta, a
connective tissue disorder characterized by bone fragility and low
bone mass.
Product Categories/Family for anti-CRTAP antibody
References
Morello, R., et al. Curr Osteoporos Rep 8(2):77-83(2010)
Li, G.H., et al. Osteoporos Int 21(6):1009-1020(2010)
Fratzl-Zelman, N., et al. Bone 46(3):820-826(2010)
Chang, W., et al. Hum. Mol. Genet. 19(2):223-234(2010)
Marini, J.C., et al. Cell Tissue Res. 339(1):59-70(2010)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
46562
NCBI Official Full Name
cartilage-associated protein
NCBI Official Synonym Full Names
cartilage associated protein
NCBI Official Symbol
CRTAP
NCBI Official Synonym Symbols
OI7; CASP; P3H5; LEPREL3
NCBI Protein Information
cartilage-associated protein
UniProt Protein Name
Cartilage-associated protein
UniProt Gene Name
CRTAP
UniProt Synonym Gene Names
CASP
UniProt Entry Name
CRTAP_HUMAN

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Product Notes

The CRTAP crtap (Catalog #AAA289096) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 31-59. The CRTAP Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CRTAP can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA, IF (Immunofluorescence). Researchers should empirically determine the suitability of the CRTAP crtap for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CRTAP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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