Rabbit anti-Human, mouse Cry2 Polyclonal Antibody | anti-CRY2 antibody

Cry2 Antibody (C-term)

Cat. Num. AAA289507

• Gene Names: CRY2; HCRY2; PHLL2
• Reactivity: Human, mouse
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: Cry2, Antibody; Cry2 Antibody (C-term); Cryptochrome-2; CRY2; KIAA0658; anti-CRY2 antibody

• Host: Rabbit
• Reactivity: Human, mouse
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This Cry2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 564-593 amino acids from the C-terminal region of human Cry2.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: Vial Concentration: 2 (varies by lot)
• Sequence Positions: 564-593
• Sequence Length: 532

• Applicable Applications for anti-CRY2 antibody: WB (Western Blot), IHC (Immunohistochemistry), ELISA
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

IHC (Immunohistochemistry)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

WB (Western Blot)

(Western blot analysis of hCry2-R579 in mouse liver tissue and HepG2 cell line lysates (35ug/lane). CRY2 (arrow) was detected using the purified Pab.)

WB (Western Blot)

(Western blot analysis of lysates from human heart, skeletal muscle and liver tissue lysate (from left to right), using Cry2 Antibody(R579). AAA289507 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)

WB (Western Blot)

(Western blot analysis of lysates from human skeletal muscle and human heart tissue (from left to right), using Cry2 Antibody (R579). AAA289507 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)

Related Product Information for anti-CRY2 antibody

Various biochemical, physiological and behavioural processes display circadian rhythms controlled by an internal biological clock. The central ?gears?driving this clock appear to be composed of an autoregulatory transcription/posttranslation-based feedback loop. Cryptochrome 1 (CRY1) and 2 (CRY2) are DNA-binding flavoproteins that bear some homology to blue-light receptors and photolyases. In Drosophila, CRY is a photoreceptor for the circadian clock where it binds to the clock component TIM in a light-dependent fashion and blocks its function. Mammalian CRY1 and CRY2 function via light-independent interactions with circadian genes CLOCK and BMAL1, as well as with PER1, PER2, and TIM. They seem to act as light-independent components of the circadian clock and likely regulate Per1 transcriptional cycling via interactions with both the activator and its feedback inhibitors. Mutant mice not expressing the Cry1 or Cry2 protein display accelerated and delayed periodicity of locomotor activity, respectively. It appears that the combination of both proteins working together is essential to synchronize the organism to circadian phases. A critical balance between Cry1 and Cry2 is required for proper clock function; in complete darkness, double-mutant mice present with instantaneous arrhythmicity, indicating the absence of an internal circadian clock.

Product Categories/Family for anti-CRY2 antibody

Cardiovascular; Neuroscience

References

Ozgur, S., et al., Biochemistry 42(10):2926-2932 (2003).
Griffin, E.A. Jr., et al., Science 286(5440):768-771 (1999).
van der Horst, G.T., et al., Nature 398(6728):627-630 (1999).
Kobayashi, K., et al., Nucleic Acids Res. 26(22):5086-5092 (1998).
Hsu, D.S., et al., Biochemistry 35(44):13871-13877 (1996).

NCBI and Uniprot Product Information

• NCBI GI #: 188536103
• NCBI GeneID: 1408
• NCBI Accession #: NP_001120929.1; NP_066940.2
• NCBI GenBank Nucleotide #: NM_001127457.2
• UniProt Accession #: Q49AN0; O75148; Q8IV71; B4DH32; B4DZD6
• Molecular Weight: 66947
• NCBI Official Full Name: cryptochrome-2 isoform 2
• NCBI Official Synonym Full Names: cryptochrome circadian clock 2
• NCBI Official Symbol: CRY2
• NCBI Official Synonym Symbols: HCRY2; PHLL2
• NCBI Protein Information: cryptochrome-2
• UniProt Protein Name: Cryptochrome-2
• UniProt Gene Name: CRY2
• UniProt Synonym Gene Names: KIAA0658
• UniProt Entry Name: CRY2_HUMAN