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IHC (Immunohistchemistry)
(DAB staining on IHC-P; Samples: Human Glioma Tissue)
Rabbit Crystallin Alpha B Polyclonal Antibody | anti-CRYaB antibody
Polyclonal Antibody to Crystallin Alpha B (CRYaB)
Gene Names
CRYAB; MFM2; CRYA2; CTPP2; HSPB5; CMD1II; CTRCT16; HEL-S-101
Reactivity
Human, Mouse, Rat, Pig
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Crystallin Alpha B, Antibody; Polyclonal Antibody to Crystallin Alpha B (CRYaB); anti-CRYaB antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Pig
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against CRYaB. It has beenselected for its ability to recognize CRYaB in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MDIAIHHPWI RRPFFPFHSP SRLFDQFFGE HLLESDLFPT STSLSPFYLR PPSFLRAPSW FDTGLSEMRL EKDRFSVNLD VKHFSPEELK VKVLGDVIEV HGKHEERQDE HGFISREFHR KYRIPADVDP LTITSSLSSD
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MDIAIHHPWI RRPFFPFHSP SRLFDQFFGE HLLESDLFPT STSLSPFYLR PPSFLRAPSW FDTGLSEMRL EKDRFSVNLD VKHFSPEELK VKVLGDVIEV HGKHEERQDE HGFISREFHR KYRIPADVDP LTITSSLSSD
Applicable Applications for anti-CRYaB antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
Fragment
CRYaB (Met1~Lys175)
Organism Species
Homo sapiens (Human)
Cross Reactivity
Human, Mouse, Pig
Conjugate
No Conjugate
Immunogen
Recombinant CRYaB (Met1~Lys175) expressed in E Coli.
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
IHC (Immunohistchemistry)
(DAB staining on IHC-P; Samples: Human Glioma Tissue)
IHC (Immunohistchemistry)
(DAB staining on IHC-P; Samples: Human Glioma Tissue)
IHC (Immunohistochemistry)
(DAB staining on IHC-P; Samples: Human Brain Tissue)
IHC (Immunohistochemistry)
(DAB staining on IHC-P; Samples: Human Brain Tissue)
IHC (Immunohistochemistry)
(DAB staining on IHC-P; Samples: Human Kidney Tissue.)
IHC (Immunohistochemistry)
(DAB staining on IHC-P; Samples: Human Kidney Tissue.)
WB (Western Blot)
(Western Blot: Sample: Recombinant CRYaB, Human.)
WB (Western Blot)
(Western Blot: Sample: Recombinant CRYaB, Human.)
WB (Western Blot)
(Western Blot: Sample: Porcine Cerebrum lysate; Primary Ab: 3ug/ml Rabbit Anti-Human CRYaB Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
WB (Western Blot)
(Western Blot: Sample: Porcine Cerebrum lysate; Primary Ab: 3ug/ml Rabbit Anti-Human CRYaB Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
WB (Western Blot)
(Western Blot: Lane1: Mouse Brain Tissue; Lane2: Mouse Heart Tissue; Lane3: Porcine Brain Tissue.)
WB (Western Blot)
(Western Blot: Lane1: Mouse Brain Tissue; Lane2: Mouse Heart Tissue; Lane3: Porcine Brain Tissue.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
20,159 Da
NCBI Official Full Name
alpha-crystallin B chain isoform 1
NCBI Official Synonym Full Names
crystallin alpha B
NCBI Official Symbol
CRYAB
NCBI Official Synonym Symbols
MFM2; CRYA2; CTPP2; HSPB5; CMD1II; CTRCT16; HEL-S-101
NCBI Protein Information
alpha-crystallin B chain
UniProt Protein Name
Alpha-crystallin B chain
UniProt Gene Name
CRYAB
UniProt Synonym Gene Names
CRYA2; HSPB5; HspB5
Similar Products
Product Notes
The CRYaB cryab (Catalog #AAA20938) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Crystallin Alpha B (CRYaB) reacts with Human, Mouse, Rat, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's Crystallin Alpha B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.5-2ug/ml Immunocytochemistry in formalin fixed cells: 5-20ug/ml Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml Immunohistochemistry in paraffin section: 5-20ug/ml Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the CRYaB cryab for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MDIAIHHPWI RRPFFPFHSP SRLFDQFFGE HLLESDLFPT STSLSPFYLR PPSFLRAPSW FDTGLSEMRL EKDRFSVNLD VKHFSPEELK VKVLGDVIEV HGKHEERQDE HGFISREFHR KYRIPADVDP LTITSSLSSD. It is sometimes possible for the material contained within the vial of "Crystallin Alpha B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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