Rabbit anti-Human CSDE1/NRU Polyclonal Antibody | anti-CSDE1 antibody

Anti-CSDE1/NRU Antibody Picoband

Cat. Num. AAA126411

• Gene Names: CSDE1; UNR; D1S155E
• Reactivity: Human
• Applications: Western Blot, Immunocytochemistry, Immunohistochemistry, Immunofluorescence, Flow Cytometry, Functional Assay, ELISA
• Purity: Immunogen affinity purified.
• Synonyms: CSDE1/NRU, Antibody; Anti-CSDE1/NRU Antibody Picoband; anti-CSDE1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized; Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. (varies by lot)

• Applicable Applications for anti-CSDE1 antibody: WB (Western Blot), ICC (Immunocytochemistry), IHC (Immunohistochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry), ELISA
• Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
• Immunogen: E Coli-derived human CSDE1/NRU recombinant protein (Position: M1-Q775).
• Preparation and Storage: Store at -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months. Avoid repeated freezing and thawing.

FCM/FACS (Flow Cytometry)

(Figure 2. Flow Cytometry analysis of THP-1 cells using anti-CSDE1/NRU antibody (AAA126411).Overlay histogram showing THP-1 cells stained with AAA126411 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CSDE1/NRU Antibody (AAA126411, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

WB (Western Blot)

(Figure 1. Western blot analysis of CSDE1/NRU using anti-CSDE1/NRU antibody (AAA126411).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human MCF-7 whole cell lysates,Lane 2: human U-87MG whole cell lysates,Lane 3: human Hela whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CSDE1/NRU antigen affinity purified polyclonal antibody (#AAA126411) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CSDE1/NRU at approximately 89 kDa. The expected band size for CSDE1/NRU is at 89 kDa.)

Related Product Information for anti-CSDE1 antibody

Cold shock domain-containing protein E1 is a protein that in humans is encoded by the CSDE1 gene. Originally described as the product of an active transcription unit located upstream of the N-RAS gene, CSDE1 (cold shock domain-containing protein E1) is a highly conserved RNA-binding protein containing five cold-shock domains. CSDE1 is mostly localized in the cytoplasm where it interacts with distinct protein complexes involved in the post-transcriptional regulation of numerous mRNAs in a dynamic and complex manner. CSDE1 plays a role in maintaining the undifferentiated state of human embryonic stem cells (hESCs), and its loss results in accelerated neural differentiation and neurogenesis, partially via post-transcriptional regulation of fatty acid binding protein 7 (FABP7) and vimentin (VIM) mRNAs. In human melanoma, increased CSDE1 protein expression has been shown to modulate the levels of pro-oncogenic factors such as vimentin or RAC1 and tumor suppressors such as PTEN, thus promoting tumor invasion and metastasis.

Product Categories/Family for anti-CSDE1 antibody

Primary Antibodies, Rabbit Polyclonal Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 18204965
• NCBI GeneID: 7812
• UniProt Accession #: O75534; O94961; Q5TF04; Q5TF05; Q68DF1; Q68DI9; Q9Y2S4; A8K281; E9PGZ0; G5E9Q2
• Molecular Weight: 85,747 Da
• NCBI Official Full Name: CSDE1 protein, partial
• NCBI Official Synonym Full Names: cold shock domain containing E1
• NCBI Official Symbol: CSDE1
• NCBI Official Synonym Symbols: UNR; D1S155E
• NCBI Protein Information: cold shock domain-containing protein E1
• UniProt Protein Name: Cold shock domain-containing protein E1
• UniProt Gene Name: CSDE1
• UniProt Synonym Gene Names: D1S155E; KIAA0885; NRU; UNR