WB (Western Blot)
(Detection of human CSTF2T by western blot. Samples: Whole cell lysate from HeLa (15 and 50 ug), HEK293T (50ug), and Jurkat (50ug) cells. Antibodies: Affinity purified rabbit anti-CSTF2T antibody AAA211500 (lot AAA211500-3) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human CSTF2T/TauCSTF64 Polyclonal Antibody | anti-CSTF2T/TauCSTF64 antibody
Rabbit anti-CSTF2T/TauCSTF64 Antibody, Affinity Purified
Gene Names
CSTF2T; CstF-64T
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
CSTF2T/TauCSTF64, Antibody; Rabbit anti-CSTF2T/TauCSTF64 Antibody, Affinity Purified; cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa, tau; CstF-64T; CSTF 64 kDa subunit tau variant; tauCstF-64; cleavage stimulation factor, 3' pre-RNA, subunit 2, tau; cleavage stimulation factor subunit 2 tau variant; Cleavage stimulation factor 64 kDa subunit tau variant; cleavage stimulation factor 64 kDa subunit tau; CF-1 64 kDa subunit tau variant; CF-1 64 kDa subunit tau; CSTF 64 kDa subunit tau; cleavage stimulation factor subunit 2, tau; Cleavage stimulation factor subunit 2 tau variant; anti-CSTF2T/TauCSTF64 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-CSTF2T/TauCSTF64 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Mouse
Immunogen
Between 566 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human CSTF2T by western blot. Samples: Whole cell lysate from HeLa (15 and 50 ug), HEK293T (50ug), and Jurkat (50ug) cells. Antibodies: Affinity purified rabbit anti-CSTF2T antibody AAA211500 (lot AAA211500-3) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human CSTF2T by western blot. Samples: Whole cell lysate from HeLa (15 and 50 ug), HEK293T (50ug), and Jurkat (50ug) cells. Antibodies: Affinity purified rabbit anti-CSTF2T antibody AAA211500 (lot AAA211500-3) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human CSTF2T by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CSTF2T antibody AAA211500 (lot AAA211500-3) used for IP at 6 ug/mg lysate. CSTF2T was also immunoprecipitated by a prior lot (AAA211500-2) of this antibody. For blotting immunoprecipitated CSTF2T, AAA211500 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human CSTF2T by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CSTF2T antibody AAA211500 (lot AAA211500-3) used for IP at 6 ug/mg lysate. CSTF2T was also immunoprecipitated by a prior lot (AAA211500-2) of this antibody. For blotting immunoprecipitated CSTF2T, AAA211500 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IHC (Immunohistochemistry)
(Detection of human CSTF2T/TauCSTF64 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-CSTF2T/TauCSTF64 (Cat. No. AAA211500 Lot3) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human CSTF2T/TauCSTF64 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-CSTF2T/TauCSTF64 (Cat. No. AAA211500 Lot3) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-CSTF2T/TauCSTF64 antibody
CSTF2T, also known as tauCSTF-64, is a paralog of CstF-64, a component of the cleavage stimulation factor (CstF) that processes 3'-end formation and polyadenylation of mRNAs. The human CSTF2T/TauCSTF64 gene is located at 10q22-23, a site associated with neurological problems and male infertility. CSTF2T is expressed in all tissues and throughout meiosis in male germ cells. CstF-64 is also expressed ubiquitously; however because it is located on the X chromosome, its expression is temporarily silenced in germ cells by meiotic sex chromosome inactivation. During this inactivation, it is hypothesized that CSTF2T/TauCSTF64 can compensate for the loss of CstfF-64 expression. Recently, targeted disruption of CSTF2T/TauCSTF64 in mice has been shown to cause aberrant spermatogenesis and infertility providing more evidence for its importance in spermatogenesis.
Product Categories/Family for anti-CSTF2T/TauCSTF64 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
64,437 Da
NCBI Official Full Name
cleavage stimulation factor subunit 2 tau variant
NCBI Official Synonym Full Names
cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa, tau variant
NCBI Official Symbol
CSTF2T
NCBI Official Synonym Symbols
CstF-64T
NCBI Protein Information
cleavage stimulation factor subunit 2 tau variant; tauCstF-64; CF-1 64 kDa subunit tau; CSTF 64 kDa subunit tau; cleavage stimulation factor 64 kDa subunit tau
UniProt Protein Name
Cleavage stimulation factor subunit 2 tau variant
UniProt Gene Name
CSTF2T
UniProt Synonym Gene Names
KIAA0689; CSTF 64 kDa subunit tau variant
UniProt Entry Name
CSTFT_HUMAN
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Product Notes
The CSTF2T/TauCSTF64 cstf2t (Catalog #AAA211500) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-CSTF2T/TauCSTF64 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CSTF2T/TauCSTF64 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the CSTF2T/TauCSTF64 cstf2t for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CSTF2T/TauCSTF64, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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