Rabbit anti-Mouse, Human CTNNBIP1/ICAT Polyclonal Antibody | anti-CTNNBIP1 antibody

CTNNBIP1/ICAT Rabbit anti-Mouse Polyclonal (aa1-81) Antibody

Cat. Num. AAA163219

• Reactivity: Mouse, Human
• Applications: ELISA, Immunocytochemistry, Western Blot, Immunohistochemistry
• Purity: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Synonyms: CTNNBIP1/ICAT, Antibody; CTNNBIP1/ICAT Rabbit anti-Mouse Polyclonal (aa1-81) Antibody; PathPlus CTNNBIP1/ICAT Antibody (aa1-81); CTNNBIP1; ICAT; anti-CTNNBIP1 antibody

• Host: Rabbit
• Reactivity: Mouse, Human
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against CTNNbIP1.
• Purity/Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Form/Format: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Concentration: 0.5mg/ml (varies by lot)

• Applicable Applications for anti-CTNNBIP1 antibody: ELISA, ICC (Immunocytochemistry), WB (Western Blot), IHC (Immunohistochemistry)
• Target: Mouse CTNNBIP1/ICAT
• Immunogen: CTNNBIP1/ Icat antibody was raised against recombinant CTNNbLP1 (Met1-Gln81) expressed in E.coli
• Immunogen Type: Recombinant Protein
• Epitope: aa1-81
• Family: Catenin
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 4 degree C for frequent use. Aliquot and store at -20 degree C for 12 months.

IHC (Immunohistochemistry)

(CTNNBIP1/ICAT Antibody-Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemisry)

(CTNNBIP1/ICAT Antibody-Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohiostchemistry)

(CTNNBIP1/ICAT Antibody-Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE))

WB (Western Blot)

(CTNNBIP1/ICAT Antibody-Western blot of recombinant CTNNBIP1/ICAT. This image was taken for the unconjugated form of this product. Other forms have not been tested.)

Related Product Information for anti-CTNNBIP1 antibody

ICAT antibody is an unconjugated rabbit polyclonal antibody to ICAT (CTNNBIP1)(aa1-81) from mouse. It is reactive with human and mouse. Validated for IHC and WB.

NCBI and Uniprot Product Information

• NCBI GeneID: 56998
• NCBI Accession #: NP_064633.1
• NCBI GenBank Nucleotide #: NM_020248
• UniProt Accession #: Q9NSA3
• UniProt Protein Name: Beta-catenin-interacting protein 1
• UniProt Gene Name: CTNNBIP1
• UniProt Synonym Gene Names: ICAT
• UniProt Entry Name: CNBP1_HUMAN