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Application Data
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Rabbit CTNND1 Polyclonal Antibody | anti-CTNND1 antibody
Phospho-CTNND1 (Tyr904) Antibody
Gene Names
CTNND1; CAS; p120; CTNND; P120CAS; P120CTN; p120(CAS); p120(CTN)
Reactivity
Human, MousePredicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%)
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Synonyms
CTNND1, Antibody; Phospho-CTNND1 (Tyr904) Antibody; Cadherin associated Src substrate; Cadherin-associated Src substrate; CAS; Catenin (cadherin associated protein) delta 1; Catenin delta 1; Catenin delta; Catenin delta-1; CTND1_HUMAN; CTNND 1; CTNND; CTNND1; KIAA0384; p120; P120 CAS; p120 catenin; P120 CTN; p120 (cas); p120 (ctn); P120CAS; P120CTN; anti-CTNND1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%)
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Phospho-CTNND1 (Tyr904) Antibody detects endogenous levels of CTNND1 only when phosphorylated at Tyr904.
Tissue Specificity: Expressed in vascular endothelium. Melanocytes and melanoma cells primarily express the long isoform 1A, whereas keratinocytes express shorter isoforms, especially 3A. The shortest isoform 4A, is detected in normal keratinocytes and melanocytes, and generally lost from cells derived from squamous cell carcinomas or melanomas. The C-terminal alternatively spliced exon B is present in the p120ctn transcripts in the colon, intestine and prostate, but lost in several tumor tissues derived from these organs.
Tissue Specificity: Expressed in vascular endothelium. Melanocytes and melanoma cells primarily express the long isoform 1A, whereas keratinocytes express shorter isoforms, especially 3A. The shortest isoform 4A, is detected in normal keratinocytes and melanocytes, and generally lost from cells derived from squamous cell carcinomas or melanomas. The C-terminal alternatively spliced exon B is present in the p120ctn transcripts in the colon, intestine and prostate, but lost in several tumor tissues derived from these organs.
Purity/Purification
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-CTNND1 antibody
ELISA (Peptide ELISA), ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human CTNND1 around the phosphorylation site of Tyr904.
Conjugation
Unconjugated
Fragment
Fab fragment
Post Translational Modifications
Phosphorylated by FER and other protein-tyrosine kinases. Phosphorylated at Ser-288 by PAK5. Dephosphorylated by PTPRJ.
Subunit Structure
Belongs to a multiprotein cell-cell adhesion complex that also contains E-cadherin/CDH1, alpha-catenin/CTNNA1, beta-catenin/CTNNB1, and gamma-catenin/JUP. Component of a cadherin:catenin adhesion complex composed of at least of CDH26, beta-catenin/CTNNB1, alpha-catenin/CTNNA1 and p120 catenin/CTNND1. Binds to the C-terminal fragment of PSEN1 and mutually competes for CDH1. Interacts with ZBTB33. Interacts with GLIS2. Interacts with FER. Interacts with NANOS1 (via N-terminal region). Interacts (via N-terminus) with GNA12; the interaction regulates CDH1-mediated cell-cell adhesion. Interacts with GNA13. Interacts with CCDC85B. Interacts with PLPP3; negatively regulates the PLPP3-mediated stabilization of CTNNB1.
Similarity
A possible nuclear localization signal exists in all isoforms where Asp-626--631-Arg are deleted.ARM repeats 1 to 5 mediate interaction with cadherins.Belongs to the beta-catenin family.
Subcellular Location
Cell junction>Adherens junction. Cytoplasm. Nucleus. Cell membrane.
Note: Interaction with GLIS2 promotes nuclear translocation (By similarity). Detected at cell-cell contacts (PubMed:15240885, PubMed:17047063). NANOS1 induces its translocation from sites of cell-cell contact to the cytoplasm (PubMed:17047063). CDH1 enhances cell membrane localization (PubMed:15240885). Isoforms 4A and 1AB are excluded from the nucleus (PubMed:11896187).Nucleus. Nucleus. Nucleus.
Note: Interaction with GLIS2 promotes nuclear translocation (By similarity). Detected at cell-cell contacts (PubMed:15240885, PubMed:17047063). NANOS1 induces its translocation from sites of cell-cell contact to the cytoplasm (PubMed:17047063). CDH1 enhances cell membrane localization (PubMed:15240885). Isoforms 4A and 1AB are excluded from the nucleus (PubMed:11896187).Nucleus. Nucleus. Nucleus.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Application Data
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Application Data
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
IHC (Immunohistochemisry)
(At 1/100 staining Mouse colorectal tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohistochemisry)
(At 1/100 staining Mouse colorectal tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis of CTNND1 (Phospho-Tyr904) using IL PMA treated NIH-3T3 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
WB (Western Blot)
(Western blot analysis of CTNND1 (Phospho-Tyr904) using IL PMA treated NIH-3T3 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
WB (Western Blot)
(Western blot analysis of extracts from various samples, using Phospho-CTNND1 (Tyr904) Antibody.Lane 1: Hela cells(serum starvation treatment), blocked with antigen-specific peptides,Lane 2: Hela cells(serum starvation treatment),Lane 3: HepG2 cells(heat shock treatment).)
WB (Western Blot)
(Western blot analysis of extracts from various samples, using Phospho-CTNND1 (Tyr904) Antibody.Lane 1: Hela cells(serum starvation treatment), blocked with antigen-specific peptides,Lane 2: Hela cells(serum starvation treatment),Lane 3: HepG2 cells(heat shock treatment).)
Related Product Information for anti-CTNND1 antibody
Key regulator of cell-cell adhesion that associates with and regulates the cell adhesion properties of both C-, E- and N-cadherins, being critical for their surface stability. Beside cell-cell adhesion, regulates gene transcription through several transcription factors including ZBTB33/Kaiso2 and GLIS2, and the activity of Rho family GTPases and downstream cytoskeletal dynamics. Implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
108,170 Da
NCBI Official Full Name
catenin delta-1 isoform 1ABC
NCBI Official Synonym Full Names
catenin (cadherin-associated protein), delta 1
NCBI Official Symbol
CTNND1
NCBI Official Synonym Symbols
CAS; p120; CTNND; P120CAS; P120CTN; p120(CAS); p120(CTN)
NCBI Protein Information
catenin delta-1; p120 catenin; cadherin-associated Src substrate
UniProt Protein Name
Catenin delta-1
UniProt Gene Name
CTNND1
UniProt Synonym Gene Names
KIAA0384; CAS; p120(ctn)
UniProt Entry Name
CTND1_HUMAN
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Product Notes
The CTNND1 ctnnd1 (Catalog #AAA329799) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-CTNND1 (Tyr904) Antibody reacts with Human, Mouse Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%) and may cross-react with other species as described in the data sheet. AAA Biotech's CTNND1 can be used in a range of immunoassay formats including, but not limited to, ELISA (Peptide ELISA), ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CTNND1 ctnnd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CTNND1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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