IHC (Immunohiostchemistry)
(AAA323661 at 1/100 staining Human kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit anti-Human Cubilin Polyclonal Antibody | anti-CUBN antibody
Cubilin Antibody
Gene Names
CUBN; IFCR; MGA1; gp280
Reactivity
Human
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
Cubilin, Antibody; Cubilin Antibody; 460 kDa receptor; cubilin (intrinsic factor-cobalamin receptor); Cubilin; Cubilin precursor; Cubn; CUBN_HUMAN; IFCR; Intestinal intrinsic factor receptor; intrinsic factor B12-receptor; Intrinsic factor-cobalamin receptor; Intrinsic factor-vitamin B12 receptor; megaloblastic anemia 1; MGA1; anti-CUBN antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Cubilin antibody detects endogenous levels of total Cubilin
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
3623
Applicable Applications for anti-CUBN antibody
ELISA, IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human Cubilin
Subcellular Location
Endosome Membrane. Lysosome Membrane. Colocalizes with AMN and LRP2 in the endocytotic apparatus of epithelial cells.
Tissue Specificity
Expressed in kidney proximal tubule cells, placenta, visceral yolk-sac cells and in absorptive intestinal cells. Expressed in the epithelium of intestine and kidney.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Dog, Chicken
Similarity
Pig (92%), Bovine (92%), Horse (92%), Sheep (92%), Dog (100%), Chicken (92%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IHC (Immunohiostchemistry)
(AAA323661 at 1/100 staining Human kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohiostchemistry)
(AAA323661 at 1/100 staining Human kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot)
(Western blot analysis of Cubilin using COLO205 whole cell lysates)
WB (Western Blot)
(Western blot analysis of Cubilin using COLO205 whole cell lysates)
Related Product Information for anti-CUBN antibody
Function: Cotransporter which plays a role in lipoprotein, vitamin and iron metabolism, by facilitating their uptake. Binds to ALB, MB, Kappa and lambda-light chains, TF, hemoglobin, GC, SCGB1A1, APOA1, high density lipoprotein, and the GIF-cobalamin complex. The binding of all ligands requires calcium. Serves as important transporter in several absorptive epithelia, including intestine, renal proximal tubules and embryonic yolk sac. Interaction with LRP2 mediates its trafficking throughout vesicles and facilitates the uptake of specific ligands like GC, hemoglobin, ALB, TF and SCGB1A1. Interaction with AMN controls its trafficking to the plasma membrane and facilitates endocytosis of ligands. May play an important role in the development of the peri-implantation embryo through internalization of APOA1 and cholesterol. Binds to LGALS3 at the maternal-fetal interface.
Subunit Structure: Interacts with LRP2 in a dual-receptor complex in a calcium-dependent manner. Component of the cubam complex composed of CUBN and AMN. The cubam complex can oligomerize and form cubam trimers. Found in a complex with PID1/PCLI1, LRP1 and CUBNI. Interacts with LRP1 and PID1/PCLI1.
Post-translational Modifications: The precursor is cleaved by a trans-Golgi proteinase furin. The result is a propeptide cleaved off. N-glycosylated.
Similarity: The CUB domains 5 to 8 mediate binding to GIF and ALB. CUB domains 1 and 2 mediate interaction with LRP2.
Subunit Structure: Interacts with LRP2 in a dual-receptor complex in a calcium-dependent manner. Component of the cubam complex composed of CUBN and AMN. The cubam complex can oligomerize and form cubam trimers. Found in a complex with PID1/PCLI1, LRP1 and CUBNI. Interacts with LRP1 and PID1/PCLI1.
Post-translational Modifications: The precursor is cleaved by a trans-Golgi proteinase furin. The result is a propeptide cleaved off. N-glycosylated.
Similarity: The CUB domains 5 to 8 mediate binding to GIF and ALB. CUB domains 1 and 2 mediate interaction with LRP2.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 399 kDa
Predicted: 399 kDa
Predicted: 399 kDa
NCBI Official Full Name
cubilin
NCBI Official Synonym Full Names
cubilin
NCBI Official Symbol
CUBN
NCBI Official Synonym Symbols
IFCR; MGA1; gp280
NCBI Protein Information
cubilin
UniProt Protein Name
Cubilin
UniProt Gene Name
CUBN
UniProt Synonym Gene Names
IFCR
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Product Notes
The CUBN cubn (Catalog #AAA323661) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Cubilin Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Cubilin can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CUBN cubn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cubilin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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