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FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of SH-SY5Y cells with Cullin 3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
Rabbit anti-Human, Mouse Cullin-3 Polyclonal Antibody | anti-CUL3 antibody
Cullin-3 Antibody
Gene Names
CUL3; CUL-3; PHA2E
Reactivity
Human, Mouse
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Purity
Peptide affinity purified
Synonyms
Cullin-3, Antibody; Cullin-3 Antibody; CUL 3 antibody; Cul-3 antibody; cul3 antibody; CUL3_HUMAN antibody; Cullin-3 antibody; Cullin3 antibody; KIAA0617 antibody; PHA2E antibody; anti-CUL3 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Peptide affinity purified
Form/Format
1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-CUL3 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
Peptide.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of SH-SY5Y cells with Cullin 3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of SH-SY5Y cells with Cullin 3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
IHC (Immunohistochemisry)
(Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Cullin-3 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemisry)
(Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Cullin-3 antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Cullin-3 antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Cullin-3 antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of Cullin 3 on different lysates using anti-Cullin 3 antibody at 1/1, 000 dilution. Positive control�� Lane1: SH-SY5Y Lane2: 293T Lane3: Mouse testis tissue)
WB (Western Blot)
(Western blot analysis of Cullin 3 on different lysates using anti-Cullin 3 antibody at 1/1, 000 dilution. Positive control�� Lane1: SH-SY5Y Lane2: 293T Lane3: Mouse testis tissue)
Related Product Information for anti-CUL3 antibody
Cullin proteins comprise a distinct family of mediators that participate in the selective targeting of proteins for ubiquitin (Ub)-mediated proteolysis. CUL-1, which is the mammalian homolog of yeast Cdc53, is an integral component of the E3 ubiquitin ligase complex designated SCF. The SCF (Skp1/CUL-1/F-box protein complex) consists of Skp1 associating with both CUL-1 and an F-box protein, such as Skp2, which determines the substrate specificity of the complex. CUL-1-mediated ubiquitination results in the degradation of cell cycle proteins cyclin D, p21 and cyclin E. Another cullin, CUL-3, facilitates the degradation of cyclin E independent of SCF activity, while CUL-2 associates with the tumor suppressing protein VHL and elongin B to form VBC complexes, which structurally resemble the SCF ligase. Proteolysis also occurs by way of CUL-4 associating with Nedd-8, a ubiquitin-like protein, where it too functions as an active component of a multifunctional E3 complex. CUL-5, also designated vasopressin-activated, calcium-mobilizing protein (VACM-1), is also included in the cullin family as it shares substantial sequence homology with CUL-1.
Product Categories/Family for anti-CUL3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
768
NCBI Official Full Name
Cullin-3
NCBI Official Synonym Full Names
cullin 3
NCBI Official Symbol
CUL3
NCBI Official Synonym Symbols
CUL-3; PHA2E
NCBI Protein Information
cullin-3
UniProt Protein Name
Cullin-3
UniProt Gene Name
CUL3
UniProt Synonym Gene Names
KIAA0617; CUL-3
UniProt Entry Name
CUL3_HUMAN
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Product Notes
The CUL3 cul3 (Catalog #AAA312103) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Cullin-3 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Cullin-3 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CUL3 cul3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cullin-3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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