Rabbit anti-Human CXCR3 Polyclonal Antibody | anti-CXCR3 antibody

Rabbit Polyclonal (IgG) to Human CXCR3

Cat. Num. AAA52142

• Gene Names: CXCR3; GPR9; MigR; CD182; CD183; Mig-R; CKR-L2; CMKAR3; IP10-R
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Immunoaffinity Purified
• Synonyms: CXCR3, Antibody; Rabbit Polyclonal (IgG) to Human CXCR3; Anti-CXCR3 Antibody (aa161-210) IHC-plus; CXCR3; C-x-c chemokine receptor 3; CD183 antigen; CKR-L2; CXCR-3; CMKAR3; CD182; CD183; Cxcr3a; GPR9; IP-10 receptor; IP10-R; Mig receptor; MigR; Mig-R; Chemokine (C-X-C) receptor 3; CXC-R3; Cxcr3b; G protein-coupled receptor 9; IP10 receptor; Human CXCR3; anti-CXCR3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CXCR3 Antibody detects endogenous levels of total CXCR3 protein.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
• Concentration: 1 mg/ml (varies by lot)

• Applicable Applications for anti-CXCR3 antibody: ELISA (Peptide ELISA), WB (Western Blot), IHC (Immunohistochemistry)
• Family/Subfamily: GPCR/Chemokine
• Immunogen Species: CXCR3 antibody was raised against Human
• Immunogen: CXCR3 antibody was raised against synthetic peptide from human CXCR3. (AA Range: 161-210)
• Epitope: aa161-210
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

Product Categories/Family for anti-CXCR3 antibody

Family: GPCR; Subfamily: Chemokine

NCBI and Uniprot Product Information

• NCBI GI #: 4504099
• NCBI GeneID: 2833
• NCBI Accession #: NP_001495.1
• NCBI GenBank Nucleotide #: NM_001504.1
• UniProt Accession #: P49682; O15185; Q7Z710; Q9P2T4; Q9P2T5; B2R982
• Molecular Weight: Western Blot: Predicted MW 40 kDa
• NCBI Official Full Name: C-X-C chemokine receptor type 3 isoform 1
• NCBI Official Synonym Full Names: chemokine (C-X-C motif) receptor 3
• NCBI Official Symbol: CXCR3
• NCBI Official Synonym Symbols: GPR9; MigR; CD182; CD183; Mig-R; CKR-L2; CMKAR3; IP10-R
• NCBI Protein Information: C-X-C chemokine receptor type 3; CXC-R3; CXCR-3; Mig receptor; IP10 receptor; IP-10 receptor; G protein-coupled receptor 9; chemokine (C-X-C) receptor 3; interferon-inducible protein 10 receptor
• UniProt Protein Name: C-X-C chemokine receptor type 3
• UniProt Gene Name: CXCR3
• UniProt Synonym Gene Names: GPR9; CXC-R3; CXCR-3; IP-10 receptor
• UniProt Entry Name: CXCR3_HUMAN