ICC (Immunocytochemistry)
(Immunocytochemical labeling of CXCR4 in chick pluripotent cells. The cells were labeled with rabbit polyclonal CXCR4 (S324/S325) antibody (CP4251), then detected using appropriate secondary antibody (Red). (Image provided by Dr. Yangqing Lu at the Regenerative Bioscience Center, University of Georgia).)
Rabbit CXCR4 Polyclonal Antibody | anti-CXCR4 antibody
CXCR4 (Ser-324/Ser-325), phospho-specific Antibody
Gene Names
CXCR4; FB22; HM89; LAP3; LCR1; NPYR; WHIM; CD184; LESTR; NPY3R; NPYRL; HSY3RR; NPYY3R; D2S201E
Reactivity
Human, Rat, Mouse, Chicken
Applications
Immunohistochemistry, Immunocytochemistry, ELISA, Western Blot
Synonyms
CXCR4, Antibody; CXCR4 (Ser-324/Ser-325), phospho-specific Antibody; CXCR4 (Ser-324/Ser-325), phospho-specific; CXCR4 Ser324; anti-CXCR4 antibody
Host
Rabbit
Reactivity
Human, Rat, Mouse, Chicken
Clonality
Polyclonal
Specificity
The antibody detects bands at 35, 42, 50, and 100kDa* corresponding to various post-translationally modified forms of CXCR4 on SDS-PAGE immunoblots of human Jurkat and PC3 cells stimulated with calyculin A. These bands co-migrate with similar bands detected with anti-CXCR4 (a.a. 322-329) and anti-CXCR4 (a.a. 328-338) antibodies.
Form/Format
Rabbit polyclonal, affinity-purified antibody is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.
Applicable Applications for anti-CXCR4 antibody
IHC (Immunohistochemistry), ICC (Immunocytochemistry), ELISA, WB (Western Blot)
Immunogen
Phospho-CXCR4 (Ser-324/Ser-325) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding Ser-324 and Ser-325 in the C-terminal region of human CXCR4. This sequence is conserved in mouse, rat, and chicken CXCR4, but has low homology to other CXCR family members.
Preparation and Storage
Store at -20 degree C. Stable for 1 year.
ICC (Immunocytochemistry)
(Immunocytochemical labeling of CXCR4 in chick pluripotent cells. The cells were labeled with rabbit polyclonal CXCR4 (S324/S325) antibody (CP4251), then detected using appropriate secondary antibody (Red). (Image provided by Dr. Yangqing Lu at the Regenerative Bioscience Center, University of Georgia).)
ICC (Immunocytochemistry)
(Immunocytochemical labeling of CXCR4 in chick pluripotent cells. The cells were labeled with rabbit polyclonal CXCR4 (S324/S325) antibody (CP4251), then detected using appropriate secondary antibody (Red). (Image provided by Dr. Yangqing Lu at the Regenerative Bioscience Center, University of Georgia).)
WB (Western Blot)
(Western blot analysis of human Jurkat cells treated with 100nM calyculin A for 30 min. then the blots were untreated (-) or treated (+) with lambda phosphatase. The blots were probed with rabbit polyclonals anti-CXCR4 (Ser-324/Ser-325) (left panel) or anti-CXCR4 (a.a. 322-329) (right panel).)
WB (Western Blot)
(Western blot analysis of human Jurkat cells treated with 100nM calyculin A for 30 min. then the blots were untreated (-) or treated (+) with lambda phosphatase. The blots were probed with rabbit polyclonals anti-CXCR4 (Ser-324/Ser-325) (left panel) or anti-CXCR4 (a.a. 322-329) (right panel).)
Related Product Information for anti-CXCR4 antibody
The chemokine receptor CXCR4 is a widely expressed G protein-coupled receptor required for development, hematopoiesis, organogenesis, and vascularization. In disease, CXCR4 has been implicated in WHIM syndrome, HIV, and cancers. Regulation of CXCR4 function occurs through phosphorylation at multiple sites in the C-terminal region. These sites have been shown to be phosphorylated after CXCL12 stimulation, and involve several kinases, such as PKC and GRK kinases. After CXCL12 stimulation of HEK293 cells, Ser-324 and Ser-325 become phosphorylated by PKC and GRK6, while Ser-330 and Ser-339 are phosphorylated by only GRK6. In human astroglia cells, Ser-324 and Ser-325 are rapidly phosphorylated in endogenous CXCR4, while Ser-330 was phosphorylated with slower kinetics. In addition, arrestin binding to CXCR4 is driven by this phosphorylation of far C-terminal residues. Thus, site-specific phosphorylation of CXCR4 may be regulated by multiple kinases leading to complex regulation of CXCR4 signaling.
References
Busillo, J. M. et al. (2010) J Biol Chem. 285(10):7805.
Bhandari, D. et al. (2009) Mol Biol Cell. 20:1324.
Orsini, M.J. et al. (2000) J Biol Chem. 275(33):25876.
Orsini, M.J. et al. (1999) J Biol Chem. 274(43):31076
Bhandari, D. et al. (2009) Mol Biol Cell. 20:1324.
Orsini, M.J. et al. (2000) J Biol Chem. 275(33):25876.
Orsini, M.J. et al. (1999) J Biol Chem. 274(43):31076
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
39,746 Da
NCBI Official Full Name
C-X-C chemokine receptor type 4 isoform a
NCBI Official Synonym Full Names
chemokine (C-X-C motif) receptor 4
NCBI Official Symbol
CXCR4
NCBI Official Synonym Symbols
FB22; HM89; LAP3; LCR1; NPYR; WHIM; CD184; LESTR; NPY3R; NPYRL; HSY3RR; NPYY3R; D2S201E
NCBI Protein Information
C-X-C chemokine receptor type 4; fusin; CXC-R4; CXCR-4; CD184 antigen; SDF-1 receptor; neuropeptide Y receptor Y3; seven transmembrane helix receptor; stromal cell-derived factor 1 receptor; lipopolysaccharide-associated protein 3; seven-transmembrane-seg
UniProt Protein Name
C-X-C chemokine receptor type 4
UniProt Gene Name
CXCR4
UniProt Synonym Gene Names
CXC-R4; CXCR-4; LESTR
UniProt Entry Name
CXCR4_HUMAN
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Product Notes
The CXCR4 cxcr4 (Catalog #AAA71615) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CXCR4 (Ser-324/Ser-325), phospho-specific Antibody reacts with Human, Rat, Mouse, Chicken and may cross-react with other species as described in the data sheet. AAA Biotech's CXCR4 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ICC (Immunocytochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the CXCR4 cxcr4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CXCR4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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