FCM/FACS (Flow Cytometry)
(CYC1 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human, mouse CYC1 Polyclonal Antibody | anti-CYC1 antibody
CYC1 Antibody (C-term)
Gene Names
CYC1; UQCR4; MC3DN6
Reactivity
Human, mouse
Applications
Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
CYC1, Antibody; CYC1 Antibody (C-term); Cytochrome c1; heme protein; mitochondrial; Complex III subunit 4; Complex III subunit IV; Cytochrome b-c1 complex subunit 4; Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit; Cytochrome c-1; CYC1; anti-CYC1 antibody
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This CYC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 263-291 amino acids from the C-terminal region of human CYC1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
263-291
Sequence Length
325
Applicable Applications for anti-CYC1 antibody
FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(CYC1 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(CYC1 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(CYC1 Antibody (C-term) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the CYC1 antibody detected the CYC1 protein (arrow).)
WB (Western Blot)
(CYC1 Antibody (C-term) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the CYC1 antibody detected the CYC1 protein (arrow).)
WB (Western Blot)
(CYC1 Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the CYC1 antibody detected the CYC1 protein (arrow).)
WB (Western Blot)
(CYC1 Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the CYC1 antibody detected the CYC1 protein (arrow).)
Related Product Information for anti-CYC1 antibody
This is the heme-containing component of the cytochrome b-c1 complex, which accepts electrons from Rieske protein and transfers electrons to cytochrome c in the mitochondrial respiratory chain.
Product Categories/Family for anti-CYC1 antibody
References
Johnatty, S.E., et al. PLoS Genet. 6 (7), E1001016 (2010) :
Cummings, C., et al. Apoptosis 11(7):1121-1129(2006)
Wen, J.J., et al. Free Radic. Biol. Med. 37(12):2072-2081(2004)
An, J., et al. J. Biol. Chem. 279(18):19133-19140(2004)
Yuan, J., et al. Biochim. Biophys. Acta 1641(1):35-41(2003)
Cummings, C., et al. Apoptosis 11(7):1121-1129(2006)
Wen, J.J., et al. Free Radic. Biol. Med. 37(12):2072-2081(2004)
An, J., et al. J. Biol. Chem. 279(18):19133-19140(2004)
Yuan, J., et al. Biochim. Biophys. Acta 1641(1):35-41(2003)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35422
NCBI Official Full Name
cytochrome c1, heme protein, mitochondrial
NCBI Official Synonym Full Names
cytochrome c-1
NCBI Official Symbol
CYC1
NCBI Official Synonym Symbols
UQCR4; MC3DN6
NCBI Protein Information
cytochrome c1, heme protein, mitochondrial
UniProt Protein Name
Cytochrome c1, heme protein, mitochondrial
UniProt Gene Name
CYC1
UniProt Synonym Gene Names
Cytochrome c-1
UniProt Entry Name
CY1_HUMAN
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Product Notes
The CYC1 cyc1 (Catalog #AAA287879) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 263-291. The CYC1 Antibody (C-term) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CYC1 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the CYC1 cyc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CYC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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