IHC (Immunohiostchemistry)
(Dilution: WB: (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)Immunohistochemical analysis of Cyclin B1 (pS126) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Rabbit anti-Human, Mouse Cyclin B1 Polyclonal Antibody | anti-CCNB1 antibody
Anti-Cyclin B1 (pS126) Antibody
Gene Names
CCNB1; CCNB
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Synonyms
Cyclin B1, Antibody; Anti-Cyclin B1 (pS126) Antibody; CCNB; G2/mitotic-specific cyclin-B1; anti-CCNB1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Applicable Applications for anti-CCNB1 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohiostchemistry)
(Dilution: WB: (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)Immunohistochemical analysis of Cyclin B1 (pS126) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
IHC (Immunohiostchemistry)
(Dilution: WB: (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)Immunohistochemical analysis of Cyclin B1 (pS126) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
WB (Western Blot)
(Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)Western blot analysis of Cyclin B1 (pS126) expression in K562 (A) whole cell lysates.)
WB (Western Blot)
(Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100)Western blot analysis of Cyclin B1 (pS126) expression in K562 (A) whole cell lysates.)
Related Product Information for anti-CCNB1 antibody
Rabbit polyclonal antibody to Cyclin B1 (pS126)
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Cyclin B1. The exact sequence is proprietary.
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Cyclin B1. The exact sequence is proprietary.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
48,337 Da
NCBI Official Full Name
G2/mitotic-specific cyclin-B1
NCBI Official Synonym Full Names
cyclin B1
NCBI Official Symbol
CCNB1
NCBI Official Synonym Symbols
CCNB
NCBI Protein Information
G2/mitotic-specific cyclin-B1; G2/mitotic-specific cyclin B1
UniProt Protein Name
G2/mitotic-specific cyclin-B1
UniProt Gene Name
CCNB1
UniProt Synonym Gene Names
CCNB
UniProt Entry Name
CCNB1_HUMAN
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Product Notes
The CCNB1 ccnb1 (Catalog #AAA292253) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Cyclin B1 (pS126) Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Cyclin B1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the CCNB1 ccnb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cyclin B1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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