WB (Western Blot)
(Detection of human Cyclin C by western blot. Samples: Whole cell lysate (10 ug) from RKO, Jurkat, HEK293T, HeLa, and K-562 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Cyclin C antibody (AAA211708 lot 3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human Cyclin C Polyclonal Antibody | anti-CCNC antibody
Rabbit anti-Cyclin C Antibody, Affinity Purified
Gene Names
CCNC; CycC
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
Cyclin C, Antibody; Rabbit anti-Cyclin C Antibody, Affinity Purified; SRB11 homolog; SRB11; CycC; hSRB11; cyclin-C; Cyclin-C; cyclin C; anti-CCNC antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-CCNC antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Mouse, Rat, Chicken, Bovine
Immunogen
Between 233 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human Cyclin C by western blot. Samples: Whole cell lysate (10 ug) from RKO, Jurkat, HEK293T, HeLa, and K-562 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Cyclin C antibody (AAA211708 lot 3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human Cyclin C by western blot. Samples: Whole cell lysate (10 ug) from RKO, Jurkat, HEK293T, HeLa, and K-562 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Cyclin C antibody (AAA211708 lot 3) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human Cyclin C by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Cyclin C antibody (AAA211708 lot 3) used for IP at 6 ug per reaction. Cyclin C was also immunoprecipitated by a previous lot of this antibody (AAA211708 lot 2). For blotting immunoprecipitated Cyclin C, AAA211708 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human Cyclin C by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Cyclin C antibody (AAA211708 lot 3) used for IP at 6 ug per reaction. Cyclin C was also immunoprecipitated by a previous lot of this antibody (AAA211708 lot 2). For blotting immunoprecipitated Cyclin C, AAA211708 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IHC (Immunohistochemistry)
(Detection of human Cyclin C by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Cyclin C (AAA211708 Lot 3) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human Cyclin C by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Cyclin C (AAA211708 Lot 3) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-CCNC antibody
Cyclin C is a member of the cyclin family of regulatory proteins that associate with and activate cyclin-dependent kinases (CDKs) to regulate cell cycle progression. Cyclins exhibit periodicity in their expression and activity which is regulated transcriptionally and post-transcriptionally via phosphorylation and ubiquitination. Cyclin C associates with cyclin-dependent kinase 8 (CDK8). It is also a component of the Mediator complex, a coactivator complex that provides a scaffold to link gene-specific transcription factors to RNA polymerase II and the general transcription machinery.
Product Categories/Family for anti-CCNC antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~55 kDa
NCBI Official Full Name
Homo sapiens cyclin C (CCNC), transcript variant 1, mRNA
NCBI Official Synonym Full Names
cyclin C
NCBI Official Symbol
CCNC
NCBI Official Synonym Symbols
CycC
NCBI Protein Information
cyclin-C; hSRB11; SRB11 homolog
UniProt Protein Name
Cyclin-C
UniProt Gene Name
CCNC
UniProt Synonym Gene Names
hSRB11
UniProt Entry Name
CCNC_HUMAN
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Product Notes
The CCNC ccnc (Catalog #AAA211708) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Cyclin C Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Cyclin C can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the CCNC ccnc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cyclin C, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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