KO (Knockout Validation)
(Knockout Validation: Lane 1: Wild-type A549 cell lysate; Lane 2: CYPD knockout A549 cell lysate; Predicted MW: 41kd Observed MW: 43kd Primary Ab: 3ug/ml Rabbit Anti-Human CYPD Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody ( Human.)
Rabbit Cyclophilin D (CYPD) Polyclonal Antibody | anti-CYPD antibody
Polyclonal Antibody to Cyclophilin D (CYPD)
Gene Names
PPID; CYPD; CYP-40
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Cyclophilin D (CYPD), Antibody; Polyclonal Antibody to Cyclophilin D (CYPD); anti-CYPD antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against CYPD. It has been selected for its ability to recognize CYPD in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- QAKP SNPSNPRVFF DVDIGGERVG RIVLELFADI VPKTAENFRA LCTGEKGIGH TTGKPLHFKG CPFHRIIKKF MIQGGDFSNQ NGTGGESIYG EKFEDENFHY KHDREGLLSM ANAGRNTNGS QFFITTVPTP HLDGKHVVFG QVIKGIGVAR ILENVEVKGE KPAKLCVIAE CGELKEGDDG GIFPKDGSGD
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- QAKP SNPSNPRVFF DVDIGGERVG RIVLELFADI VPKTAENFRA LCTGEKGIGH TTGKPLHFKG CPFHRIIKKF MIQGGDFSNQ NGTGGESIYG EKFEDENFHY KHDREGLLSM ANAGRNTNGS QFFITTVPTP HLDGKHVVFG QVIKGIGVAR ILENVEVKGE KPAKLCVIAE CGELKEGDDG GIFPKDGSGD
Applicable Applications for anti-CYPD antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant CYPD (Gln7~Pro206) expressed in E.coli.
Cross Reactivity
Human, Mouse, Rat
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
KO (Knockout Validation)
(Knockout Validation: Lane 1: Wild-type A549 cell lysate; Lane 2: CYPD knockout A549 cell lysate; Predicted MW: 41kd Observed MW: 43kd Primary Ab: 3ug/ml Rabbit Anti-Human CYPD Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody ( Human.)
KO (Knockout Validation)
(Knockout Validation: Lane 1: Wild-type A549 cell lysate; Lane 2: CYPD knockout A549 cell lysate; Predicted MW: 41kd Observed MW: 43kd Primary Ab: 3ug/ml Rabbit Anti-Human CYPD Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody ( Human.)
WB (Western Blot)
(Western Blot: Sample: Lane1: Human A549 Cells; Lane2: Mouse Spleen Tissue; Lane3: Mouse Brain Tissue; Lane4: Rat Brain Tissue.)
WB (Western Blot)
(Western Blot: Sample: Lane1: Human A549 Cells; Lane2: Mouse Spleen Tissue; Lane3: Mouse Brain Tissue; Lane4: Rat Brain Tissue.)
Product Categories/Family for anti-CYPD antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
40,764 Da
NCBI Official Full Name
peptidyl-prolyl cis-trans isomerase D
NCBI Official Synonym Full Names
peptidylprolyl isomerase D
NCBI Official Symbol
PPID
NCBI Official Synonym Symbols
CYPD; CYP-40
NCBI Protein Information
peptidyl-prolyl cis-trans isomerase D
UniProt Protein Name
Peptidyl-prolyl cis-trans isomerase D
UniProt Gene Name
PPID
UniProt Synonym Gene Names
CYP40; CYPD; PPIase D; CYP-40
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Product Notes
The CYPD ppid (Catalog #AAA20302) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Cyclophilin D (CYPD) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Cyclophilin D (CYPD) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the CYPD ppid for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- QAKP SNPSNPRVFF DVDIGGERVG RIVLELFADI VPKTAENFRA LCTGEKGIGH TTGKPLHFKG CPFHRIIKKF MIQGGDFSNQ NGTGGESIYG EKFEDENFHY KHDREGLLSM ANAGRNTNGS QFFITTVPTP HLDGKHVVFG QVIKGIGVAR ILENVEVKGE KPAKLCVIAE CGELKEGDDG GIFPKDGSGD. It is sometimes possible for the material contained within the vial of "Cyclophilin D (CYPD), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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