IF (Immunofluorescence)
(Immunofluorescence staining of S3-PPIF in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human S3-PPIF polyclonal antibody (dilution ratio 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm.)
Rabbit anti-Human Cyclophilin F Polyclonal Antibody | anti-PPIF antibody
Anti-Cyclophilin F Antibody, Rabbit Polyclonal
Gene Names
PPIF; CYP3; CypD; CyP-M; Cyp-D
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Protein A & Antigen Affinity
Synonyms
Cyclophilin F, Antibody; Anti-Cyclophilin F Antibody, Rabbit Polyclonal; PPIF/Cyclophilin F Antibody, Rabbit PAb, Antigen Affinity Purified; peptidylprolyl isomerase F; Anti-Cyp-D Antibody; Anti-CyP-M Antibody; Anti-CYP3 Antibody; Anti-CypD Antibody; anti-PPIF antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human PPIF/Cyclophilin F
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-PPIF antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry), ELISA
Immunogen
Recombinant Human PPIF/Cyclophilin F Protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human PPIF/Cyclophilin F (rh PPIF/Cyclophilin F). PPIF/Cyclophilin F specific IgG was purified by Human PPIF/Cyclophilin F affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence staining of S3-PPIF in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human S3-PPIF polyclonal antibody (dilution ratio 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm.)
IF (Immunofluorescence)
(Immunofluorescence staining of S3-PPIF in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human S3-PPIF polyclonal antibody (dilution ratio 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor$488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm.)
IHC (Immunohistochemistry)
(Immunochemical staining of human PPIF in human colon carcinoma with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry)
(Immunochemical staining of human PPIF in human colon carcinoma with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.)
Product Categories/Family for anti-PPIF antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22,040 Da
NCBI Official Full Name
peptidyl-prolyl cis-trans isomerase F, mitochondrial
NCBI Official Synonym Full Names
peptidylprolyl isomerase F
NCBI Official Symbol
PPIF
NCBI Official Synonym Symbols
CYP3; CypD; CyP-M; Cyp-D
NCBI Protein Information
peptidyl-prolyl cis-trans isomerase F, mitochondrial; PPIase F; rotamase F; cyclophilin 3; cyclophilin D; cyclophilin F; mitochondrial cyclophilin; peptidyl-prolyl cis-trans isomerase, mitochondrial
UniProt Protein Name
Peptidyl-prolyl cis-trans isomerase F, mitochondrial
UniProt Gene Name
PPIF
UniProt Synonym Gene Names
CYP3; PPIase F; CyP-D; CypD; CyP-M
UniProt Entry Name
PPIF_HUMAN
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Product Notes
The PPIF ppif (Catalog #AAA255791) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Cyclophilin F Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Cyclophilin F can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the PPIF ppif for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cyclophilin F, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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