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IHC (Immunohistochemistry)
(Anti- CYP1A2 Picoband antibody, AAA46318, IHC(P)IHC(P): Human Liver Cancer Tissue)
CYP1A2 Polyclonal Antibody | anti-CYP1A2 antibody
Anti-CYP1A2 Antibody
Gene Names
CYP1A2; CP12; P3-450; P450(PA)
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen Affinity Purified
Synonyms
CYP1A2, Antibody; Anti-CYP1A2 Antibody; Cytochrome P450 1A2; Aryl hydrocarbon hydroxylase; CP 12; CP12; CP1A2_HUMAN; CYP1A2; CYPIA2; Cytochrome P(3)450; Cytochrome P450 4; Cytochrome P450 family 1 polypeptide 2; Cytochrome P450 family 1 subfamily A polypeptide 2; Cytochrome P450 subfamily I aromatic compound inducible polypeptide 2; Cytochrome P450-P3; Cytochrome P4501A2; Dioxin inducable P3 450; Flavoprotein linked monooxygenase; Microsomal monooxygenase; P(3)450; P3 450; P450 4; P450 form 4; P450 P3; P450(PA); Xenobiotic monooxygenase antibody; cytochrome P450, family 1, subfamily A, polypeptide 2; anti-CYP1A2 antibody
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Purity/Purification
Immunogen Affinity Purified
Form/Format
Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
516
Applicable Applications for anti-CYP1A2 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
E Coli-derived human CYP1A2 recombinant protein (Position: H185-D320). Human CYP1A2 shares 69.9% and 72.1% amino acid (aa) sequence identity with mouse and rat CYP1A2, respectively.
Ig Type
Rabbit IgG
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
IHC (Immunohistochemistry)
(Anti- CYP1A2 Picoband antibody, AAA46318, IHC(P)IHC(P): Human Liver Cancer Tissue)
IHC (Immunohistochemistry)
(Anti- CYP1A2 Picoband antibody, AAA46318, IHC(P)IHC(P): Human Liver Cancer Tissue)
IHC (Immunohistochemisry)
(Anti- CYP1A2 Picoband antibody, AAA46318, IHC(P)IHC(P): Rat Liver Tissue)
IHC (Immunohistochemisry)
(Anti- CYP1A2 Picoband antibody, AAA46318, IHC(P)IHC(P): Rat Liver Tissue)
IHC (Immunohiostchemistry)
(Anti- CYP1A2 Picoband antibody, AAA46318, IHC(P)IHC(P): Mouse Liver Tissue)
IHC (Immunohiostchemistry)
(Anti- CYP1A2 Picoband antibody, AAA46318, IHC(P)IHC(P): Mouse Liver Tissue)
WB (Western Blot)
(Anti- CYP1A2 Picoband antibody, AAA46318, Western blottingAll lanes: Anti CYP1A2 (AAA46318) at 0.5ug/mlLane 1: Rat Liver Tissue Lysate at 50ugLane 2: Mouse Liver Tissue Lysate at 50ugPredicted bind size: 58KDObserved bind size: 58KD)
WB (Western Blot)
(Anti- CYP1A2 Picoband antibody, AAA46318, Western blottingAll lanes: Anti CYP1A2 (AAA46318) at 0.5ug/mlLane 1: Rat Liver Tissue Lysate at 50ugLane 2: Mouse Liver Tissue Lysate at 50ugPredicted bind size: 58KDObserved bind size: 58KD)
Related Product Information for anti-CYP1A2 antibody
Description: Rabbit IgG polyclonal antibody for Cytochrome P450 1A2(CYP1A2) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Background: CYP1A2 (Cytochrome P450, Subfamily I, Polypeptide 2) is a member of the cytochrome P450 mixed-function oxidase system and is involved in the metabolism of xenobiotics in the body. CYP1A2 is a member of the cytochrome P450 superfamily of enzymes. In humans, the CYP1A2 enzyme is encoded by the CYP1A2 gene. CYP1A2 localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The CYP1A2 gene encodes a P450 enzyme involved in O-deethylation of phenacetin. Ikeya et al. (1989) found that the human CYP1A2 gene spans almost 7.8 kb and contains 7 exons. The CYP1A2 gene is mapped on 15q24.1. CYP1A2 accounts for nearly 15% of the cytochrome P450 in the human liver. CYP1A2 displays higher activity in men than in women, and is inhibited by oral contraceptives. Inducers of CYP1A2 include cruciferous vegetables. Cigarette smoking has also been shown to increase CYP1A2 activity. Expression of CYP1A2 appears to be induced by various dietary constituents.
Background: CYP1A2 (Cytochrome P450, Subfamily I, Polypeptide 2) is a member of the cytochrome P450 mixed-function oxidase system and is involved in the metabolism of xenobiotics in the body. CYP1A2 is a member of the cytochrome P450 superfamily of enzymes. In humans, the CYP1A2 enzyme is encoded by the CYP1A2 gene. CYP1A2 localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The CYP1A2 gene encodes a P450 enzyme involved in O-deethylation of phenacetin. Ikeya et al. (1989) found that the human CYP1A2 gene spans almost 7.8 kb and contains 7 exons. The CYP1A2 gene is mapped on 15q24.1. CYP1A2 accounts for nearly 15% of the cytochrome P450 in the human liver. CYP1A2 displays higher activity in men than in women, and is inhibited by oral contraceptives. Inducers of CYP1A2 include cruciferous vegetables. Cigarette smoking has also been shown to increase CYP1A2 activity. Expression of CYP1A2 appears to be induced by various dietary constituents.
References
1. Browning, S. L., Tarekegn, A., Bekele, E., Bradman, N., Thomas, M. G. CYP1A2 is more variable than previously thought: a genomic biography of the gene behind the human drug-metabolizing enzyme. Pharmacogenet. Genomics 20: 647-664, 2010. 2. Ikeya, K., Jaiswal, A. K., Owens, R. A., Jones, J. E., Nebert, D. W., Kimura, S. Human CYP1A2: sequence, gene structure, comparison with the mouse and rat orthologous gene, and differences in liver 1A2 mRNA expression. Molec. Endocr. 3: 1399-1408, 1989. 3. Nakajima, M., Yokoi, T., Mizutani, M., Kinoshita, M., Funayama, M., Kamataki, T. Genetic polymorphism in the 5-prime-flanking region of human CYP1A2 gene: effect on theCYP1A2 inducibility in humans. J. Biochem. 125: 803-808, 1999.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
58,407 Da
NCBI Official Full Name
cytochrome P450 1A2
NCBI Official Synonym Full Names
cytochrome P450 family 1 subfamily A member 2
NCBI Official Symbol
CYP1A2
NCBI Official Synonym Symbols
CP12; P3-450; P450(PA)
NCBI Protein Information
cytochrome P450 1A2
UniProt Protein Name
Cytochrome P450 1A2
UniProt Gene Name
CYP1A2
UniProt Entry Name
CP1A2_HUMAN
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Product Notes
The CYP1A2 cyp1a2 (Catalog #AAA46318) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-CYP1A2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CYP1A2 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CYP1A2 cyp1a2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CYP1A2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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