WB (Western Blot)
(All lanes : Anti-CYP24A1 Antibody (C-term) at 1:1000 dilutionLane 1: A431 whole cell lysatesLane 2: Hela whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 59 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human CYP24A1 Polyclonal Antibody | anti-CYP24A1 antibody
CYP24A1 Antibody (C-term)
Gene Names
CYP24A1; CP24; HCAI; CYP24; P450-CC24
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
CYP24A1, Antibody; CYP24A1 Antibody (C-term); 25-dihydroxyvitamin D(3) 24-hydroxylase; mitochondrial; 24-OHase; Vitamin D(3) 24-hydroxylase; Cytochrome P450 24A1; Cytochrome P450-CC24; CYP24A1; CYP24; anti-CYP24A1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This CYP24A1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 480-514 amino acids from the C-terminal region of human CYP24A1.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Length
372
Applicable Applications for anti-CYP24A1 antibody
ELISA, WB (Western Blot)
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(All lanes : Anti-CYP24A1 Antibody (C-term) at 1:1000 dilutionLane 1: A431 whole cell lysatesLane 2: Hela whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 59 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-CYP24A1 Antibody (C-term) at 1:1000 dilutionLane 1: A431 whole cell lysatesLane 2: Hela whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 59 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-CYP24A1 Antibody (C-term)at 1:2000 dilution + HepG2 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 59 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-CYP24A1 Antibody (C-term)at 1:2000 dilution + HepG2 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 59 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-CYP24A1 antibody
Has a role in maintaining calcium homeostasis. Catalyzes the NADPH-dependent 24-hydroxylation of calcidiol (25- hydroxyvitamin D(3)) and calcitriol (1-alpha,25-dihydroxyvitamin D(3)). The enzyme can perform up to 6 rounds of hydroxylation of calcitriol leading to calcitroic acid. It also shows 23- hydroxylating activity leading to 1-alpha,25-dihydroxyvitamin D(3)-26,23-lactone as end product.
References
Chen K.-S.,et al.Proc. Natl. Acad. Sci. U.S.A. 90:4543-4547(1993).
Ren S.,et al.J. Biol. Chem. 280:20604-20611(2005).
Deloukas P.,et al.Nature 414:865-871(2001).
Chen K.-S.,et al.Biochim. Biophys. Acta 1263:1-9(1995).
Labuda M.,et al.J. Bone Miner. Res. 8:1397-1406(1993).
Ren S.,et al.J. Biol. Chem. 280:20604-20611(2005).
Deloukas P.,et al.Nature 414:865-871(2001).
Chen K.-S.,et al.Biochim. Biophys. Acta 1263:1-9(1995).
Labuda M.,et al.J. Bone Miner. Res. 8:1397-1406(1993).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
58875
NCBI Official Full Name
1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial isoform 1
NCBI Official Synonym Full Names
cytochrome P450, family 24, subfamily A, polypeptide 1
NCBI Official Symbol
CYP24A1
NCBI Official Synonym Symbols
CP24; HCAI; CYP24; P450-CC24
NCBI Protein Information
1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial
UniProt Protein Name
1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial
UniProt Gene Name
CYP24A1
UniProt Synonym Gene Names
CYP24; 24-OHase; Vitamin D(3) 24-hydroxylase
UniProt Entry Name
CP24A_HUMAN
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Product Notes
The CYP24A1 cyp24a1 (Catalog #AAA284999) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CYP24A1 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CYP24A1 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the CYP24A1 cyp24a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CYP24A1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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