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product-image-AAA285535_IF11.jpg IF (Immunofluorescence) (Confocal immunofluorescent analysis of CYP3A43 Antibody (Center) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Rabbit anti-Human, mouse CYP3A43 Polyclonal Antibody | anti-CYP3A43 antibody

CYP3A43 Antibody (Center)

Reactivity
Human, mouse
Applications
Flow Cytometry, Functional Assay, Western Blot, ELISA, Immunofluorescence
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
CYP3A43, Antibody; CYP3A43 Antibody (Center); Cytochrome P450 3A43; CYP3A43; anti-CYP3A43 antibody
Ordering
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This CYP3A43 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 242-270 amino acids from the Central region of human CYP3A43.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
242-270
Sequence Length
393
Applicable Applications for anti-CYP3A43 antibody
FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA, IF (Immunofluorescence)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

IF (Immunofluorescence)

(Confocal immunofluorescent analysis of CYP3A43 Antibody (Center) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

product-image-AAA285535_IF11.jpg IF (Immunofluorescence) (Confocal immunofluorescent analysis of CYP3A43 Antibody (Center) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

FCM/FACS (Flow Cytometry)

(CYP3A43 Antibody (Center) flow cytometry analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

product-image-AAA285535_FCM13.jpg FCM/FACS (Flow Cytometry) (CYP3A43 Antibody (Center) flow cytometry analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

WB (Western Blot)

(Western blot analysis of CYP3A43 antibody (Center) in mouse brain tissue lysates (35ug/lane). CYP3A43 (arrow) was detected using the purified Pab.)

product-image-AAA285535_WB15.jpg WB (Western Blot) (Western blot analysis of CYP3A43 antibody (Center) in mouse brain tissue lysates (35ug/lane). CYP3A43 (arrow) was detected using the purified Pab.)
Related Product Information for anti-CYP3A43 antibody
CYP3A43 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein has a low level of testosterone hydroxylase activity. Although it bears homology to some drug-metabolizing cytochrome P450s, the protein unknown whether the enzyme is also involved in xenobiotic metabolism.
Product Categories/Family for anti-CYP3A43 antibody
References
Domanski T.L., Finta C.Mol. Pharmacol. 59:386-392(2001)
Westlind A., Malmebo S.Biochem. Biophys. Res. Commun. 281:1349-1355(2001)
Cauffiez C., Lo-Guidice J.-M.Hum. Mutat. 23:101-101(2004)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
57670
NCBI Official Full Name
cytochrome P450 3A43 isoform 4
NCBI Official Synonym Full Names
cytochrome P450, family 3, subfamily A, polypeptide 43
NCBI Official Symbol
CYP3A43
NCBI Protein Information
cytochrome P450 3A43
UniProt Protein Name
Cytochrome P450 3A43
UniProt Gene Name
CYP3A43
UniProt Entry Name
CP343_HUMAN

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Product Notes

The CYP3A43 cyp3a43 (Catalog #AAA285535) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 242-270. The CYP3A43 Antibody (Center) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CYP3A43 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA, IF (Immunofluorescence). Researchers should empirically determine the suitability of the CYP3A43 cyp3a43 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CYP3A43, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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