FCM/FACS (Flow Cytometry)
(CYP4F3 Antibody (N-term) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human CYP4F3 Polyclonal Antibody | anti-CYP4F3 antibody
CYP4F3 Antibody (N-term)
Gene Names
CYP4F3; CPF3; CYP4F; LTB4H
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
CYP4F3, Antibody; CYP4F3 Antibody (N-term); Docosahexaenoic acid omega-hydroxylase CYP4F3; 20-hydroxyeicosatetraenoic acid synthase; 20-HETE synthase; 11413-; CYPIVF3; Cytochrome P450 4F3; Cytochrome P450-LTB-omega; Leukotriene-B(4) 20-monooxygenase 2; Leukotriene-B(4) omega-hydroxylase 2; CYP4F3; LTB4H; anti-CYP4F3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This CYP4F3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 77-109 amino acids from the N-terminal region of human CYP4F3.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
77-109
Sequence Length
520
Applicable Applications for anti-CYP4F3 antibody
FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(CYP4F3 Antibody (N-term) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(CYP4F3 Antibody (N-term) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(Western blot analysis of CYP4F3 Antibody (N-term) in K562 cell line lysates (35ug/lane). CYP4F3 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of CYP4F3 Antibody (N-term) in K562 cell line lysates (35ug/lane). CYP4F3 (arrow) was detected using the purified Pab.)
Related Product Information for anti-CYP4F3 antibody
CYP4F3 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. The enzyme starts the process of inactivating and degrading leukotriene B4, a potent mediator of inflammation.
Product Categories/Family for anti-CYP4F3 antibody
References
Kikuta,Y., et.al., FEBS Lett. 348 (1), 70-74 (1994)
Kikuta,Y., et,al., DNA Cell Biol. 17 (3), 221-230 (1998)
Kikuta,Y., et,al., DNA Cell Biol. 17 (3), 221-230 (1998)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
59847
NCBI Official Full Name
docosahexaenoic acid omega-hydroxylase CYP4F3 isoform a
NCBI Official Synonym Full Names
cytochrome P450, family 4, subfamily F, polypeptide 3
NCBI Official Symbol
CYP4F3
NCBI Official Synonym Symbols
CPF3; CYP4F; LTB4H
NCBI Protein Information
docosahexaenoic acid omega-hydroxylase CYP4F3
UniProt Protein Name
Docosahexaenoic acid omega-hydroxylase CYP4F3
UniProt Gene Name
CYP4F3
UniProt Synonym Gene Names
LTB4H; 20-HETE synthase
UniProt Entry Name
CP4F3_HUMAN
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Product Notes
The CYP4F3 cyp4f3 (Catalog #AAA290410) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 77-109. The CYP4F3 Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CYP4F3 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the CYP4F3 cyp4f3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CYP4F3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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