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IF (Immunofluorescence)
(AAA324272 staining Hela cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Rabbit Cytochrome P450 3A4/5 Polyclonal Antibody | anti-CYP3A4 antibody
Cytochrome P450 3A4/5 Antibody
Gene Names
CYP3A4; HLP; CP33; CP34; CYP3A; NF-25; CYP3A3; P450C3; CYPIIIA3; CYPIIIA4; P450PCN1
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
Cytochrome P450 3A4/5, Antibody; Cytochrome P450 3A4/5 Antibody; 1, 8-cineole 2-exo-monooxygenase; Albendazole monooxygenase; Albendazole sulfoxidase; CP33; CP34; CP3A4_HUMAN; CYP3; CYP3A; CYP3A3; CYP3A4; CYPIIIA3; CYPIIIA4; Cytochrome P450 3A3; Cytochrome P450 3A4; Cytochrome P450 family 3 subfamily A polypeptide 4; Cytochrome P450 HLp; Cytochrome P450 NF-25; Cytochrome P450 subfamily IIIA polypeptide 4; cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 3; cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 4; Cytochrome P450-PCN1; Glucocorticoid inducible P450; HLP; MGC126680; NF 25; NF25; Nifedipine oxidase; P450 III steroid inducible; P450 PCN1; P450, family III; P450C3; P450PCN1; Quinine 3 monooxygenase; Quinine 3-monooxygenase; Taurochenodeoxycholate 6 alpha hydroxylase; Taurochenodeoxycholate 6-alpha-hydroxylase; Aryl hydrocarbon hydroxylase; CP35; CP3A5_HUMAN; CYP3A5; CYPIIIA5; Cytochrome P450 3A5; Cytochrome P450; Cytochrome P450 family 3 subfamily A polypeptide 5; Cytochrome P450 HLp2; Cytochrome P450 subfamily IIIA (niphedipine oxidase) polypeptide 5; Cytochrome P450-PCN3; DKFZp686L16231; Flavoprotein linked monooxygenase; FLJ31317; HLp2; Microsomal monooxygenase; Niphedipine oxidase; P450PCN3; PCN3; Xenobiotic monooxygenase; anti-CYP3A4 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Cytochrome P450 3A4/5 antibody detects endogenous levels of total Cytochrome P450 3A4/5
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
502
Applicable Applications for anti-CYP3A4 antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
Immunogen
A synthesized peptide
Subcellular Location
Endoplasmic Reticulum Membrane. Microsome Membrane.
Tissue Specificity
Expressed in prostate and liver. According to some authors, it is not expressed in brain (PubMed:19094056). According to others, weak levels of expression are measured in some brain locations (PubMed:19359404 and PubMed:18545703). Also expressed in epithelium of the small intestine and large intestine, bile duct, nasal mucosa, kidney, adrenal cortex, epithelium of the gastric mucosa with intestinal metaplasia, gallbladder, intercalated ducts of the pancreas, chief cells of the parathyroid and the corpus luteum of the ovary (at protein level).
Predicted Cross Reactivity
Bovine, Dog
Similarity
Bovine (82%), Dog (82%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IF (Immunofluorescence)
(AAA324272 staining Hela cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
IF (Immunofluorescence)
(AAA324272 staining Hela cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
WB (Western Blot)
(Western blot analysis of extracts from various samples, using Cytochrome P450 3A4/5 Antibody. Lane 1: Hela treated with blocking peptide; Lane 2: Hela; Lane 3: rat brain;)
WB (Western Blot)
(Western blot analysis of extracts from various samples, using Cytochrome P450 3A4/5 Antibody. Lane 1: Hela treated with blocking peptide; Lane 2: Hela; Lane 3: rat brain;)
WB (Western Blot)
(Western blot analysis of extracts from rat Brian, using Cytochrome P450 3A4/5 antibody.)
WB (Western Blot)
(Western blot analysis of extracts from rat Brian, using Cytochrome P450 3A4/5 antibody.)
WB (Western Blot)
(Western blot analysis of extracts from Jurkat cells, using Cytochrome P450 3A4/5 antibody.The lane on the left is treated with the antigen-specific peptide.)
WB (Western Blot)
(Western blot analysis of extracts from Jurkat cells, using Cytochrome P450 3A4/5 antibody.The lane on the left is treated with the antigen-specific peptide.)
Related Product Information for anti-CYP3A4 antibody
Function: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It performs a variety of oxidation reactions (e.g. caffeine 8-oxidation, omeprazole sulphoxidation, midazolam 1'-hydroxylation and midazolam 4-hydroxylation) of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Acts as a 1, 8-cineole 2-exo-monooxygenase. The enzyme also hydroxylates etoposide (PubMed:11159812). Catalyzes 4-beta-hydroxylation of cholesterol. May catalyze 25-hydroxylation of cholesterol in vitro (PubMed:21576599).
Post-translational Modifications: Polyubiquitinated in the presence of AMFR and UBE2G1 and also STUB1/CHIP and UBE2D1 (in vitro).
Similarity: Belongs to the cytochrome P450 family.
Post-translational Modifications: Polyubiquitinated in the presence of AMFR and UBE2G1 and also STUB1/CHIP and UBE2D1 (in vitro).
Similarity: Belongs to the cytochrome P450 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 57 kD
Predicted: 58 kDa
Predicted: 58 kDa
NCBI Official Full Name
cytochrome P450 3A4 isoform 2
NCBI Official Synonym Full Names
cytochrome P450 family 3 subfamily A member 4
NCBI Official Symbol
CYP3A4
NCBI Official Synonym Symbols
HLP; CP33; CP34; CYP3A; NF-25; CYP3A3; P450C3; CYPIIIA3; CYPIIIA4; P450PCN1
NCBI Protein Information
cytochrome P450 3A4
UniProt Protein Name
Cytochrome P450 3A5
UniProt Gene Name
CYP3A5
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Product Notes
The CYP3A4 cyp3a5 (Catalog #AAA324272) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Cytochrome P450 3A4/5 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Cytochrome P450 3A4/5 can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the CYP3A4 cyp3a5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cytochrome P450 3A4/5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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