ICC (Immunocytochemistry)
(Anti-POR antibody, AAA45447, ICCICC: HELA Cell)
Rabbit Cytochrome P450 Reductase Polyclonal Antibody | anti-POR antibody
Anti-Cytochrome P450 Reductase antibody
Gene Names
POR; CPR; CYPOR; P450R
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Synonyms
Cytochrome P450 Reductase, Antibody; Anti-Cytochrome P450 Reductase antibody; NADPH--cytochrome P450 reductase; P450(cytochrome) oxidoreductase; CPR antibody; CYPOR antibody; DKFZp686G04235 antibody; FLJ26468 antibody; NADPH Cytochrome P450 Reductase antibody; NADPH dependent cytochrome P450 reductase antibody; NADPH--cytochrome P450 reductase antibody; NCPR_HUMAN antibody; P450(cytochrome) oxidoreductase antibody; P450 Cytochrome Oxidoreductase antibody; P450R antibody; POR antibody; anti-POR antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
677
Applicable Applications for anti-POR antibody
ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Cytochrome P450 Reductase (659-677aa DYIKKLMTKGRYSLDVWS), different from the related rat and mouse sequences by one amino acid.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
ICC (Immunocytochemistry)
(Anti-POR antibody, AAA45447, ICCICC: HELA Cell)
ICC (Immunocytochemistry)
(Anti-POR antibody, AAA45447, ICCICC: HELA Cell)
IHC (Immunohiostchemistry)
(Anti-POR antibody, AAA45447, IHC(P)IHC(P): Human Placenta Tissue)
IHC (Immunohiostchemistry)
(Anti-POR antibody, AAA45447, IHC(P)IHC(P): Human Placenta Tissue)
WB (Western Blot)
(Anti-POR antibody, AAA45447, Western blottingLane 1: Rat Ovary Tissue LysateLane 2: Rat Lung Tissue LysateLane 3: Rat Testis Tissue LysateLane 4: Rat Spleen Tissue LysateLane 5: A549 Cell LysateLane 6: HELA Cell LysateLane 7: SKOV Cell LysateLane 8: MCF-7 Cell Lysate)
WB (Western Blot)
(Anti-POR antibody, AAA45447, Western blottingLane 1: Rat Ovary Tissue LysateLane 2: Rat Lung Tissue LysateLane 3: Rat Testis Tissue LysateLane 4: Rat Spleen Tissue LysateLane 5: A549 Cell LysateLane 6: HELA Cell LysateLane 7: SKOV Cell LysateLane 8: MCF-7 Cell Lysate)
Related Product Information for anti-POR antibody
Description: Rabbit IgG polyclonal antibody for NADPH--cytochrome P450 reductase(POR) detection. Tested with WB, IHC-P, ICC in Human, Mouse, Rat.
Background: POR(Cytochrome P450 Oxidoreductase), also known as Cytochrome P450 Reductase, is a membrane-bound enzyme required for electron transfer to cytochrome P450 in the endoplasmic reticulumof the eukaryotic cell from a FAD- and FMN-containing enzyme NADPH: cytochrome P450 reductase. By Southern blot analysis of DNA isolated from a panel of 8 independent human-rodent somatic cell hybrids, Shephard et al.(1989) determined that cytochrome P450 reductase is encoded by a single gene located on 7pter-q22. By in situ hybridization to metaphase chromosomes, they refined the localization to 7q11.2. Cytochrome P450 oxidoreductase is a flavoprotein that donates electrons to all microsomal P450 enzymes, including the steroidogenic enzymes P450c17(CYP17A1), P450c21(CYP21A2), and CYP51A1(Miller, 1986).
Background: POR(Cytochrome P450 Oxidoreductase), also known as Cytochrome P450 Reductase, is a membrane-bound enzyme required for electron transfer to cytochrome P450 in the endoplasmic reticulumof the eukaryotic cell from a FAD- and FMN-containing enzyme NADPH: cytochrome P450 reductase. By Southern blot analysis of DNA isolated from a panel of 8 independent human-rodent somatic cell hybrids, Shephard et al.(1989) determined that cytochrome P450 reductase is encoded by a single gene located on 7pter-q22. By in situ hybridization to metaphase chromosomes, they refined the localization to 7q11.2. Cytochrome P450 oxidoreductase is a flavoprotein that donates electrons to all microsomal P450 enzymes, including the steroidogenic enzymes P450c17(CYP17A1), P450c21(CYP21A2), and CYP51A1(Miller, 1986).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
76,690 Da
NCBI Official Full Name
NADPH--cytochrome P450 reductase
NCBI Official Synonym Full Names
P450 (cytochrome) oxidoreductase
NCBI Official Symbol
POR
NCBI Official Synonym Symbols
CPR; CYPOR; P450R
NCBI Protein Information
NADPH--cytochrome P450 reductase; NADPH-dependent cytochrome P450 reductase
UniProt Protein Name
NADPH--cytochrome P450 reductase
UniProt Gene Name
POR
UniProt Synonym Gene Names
CYPOR; CPR; P450R
UniProt Entry Name
NCPR_HUMAN
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Product Notes
The POR por (Catalog #AAA45447) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Cytochrome P450 Reductase antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Cytochrome P450 Reductase can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the POR por for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cytochrome P450 Reductase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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