WB (Western Blot)
(Detection of human Dab2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-Dab2 antibody AAA212438 (lot AAA212438-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human Dab2 Polyclonal Antibody | anti-DAB2 antibody
Rabbit anti-Dab2 Antibody, Affinity Purified
Gene Names
DAB2; DOC2; DOC-2
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
Dab2, Antibody; Rabbit anti-Dab2 Antibody, Affinity Purified; Dab, mitogen-responsive phosphoprotein, homolog 2; differentially expressed in ovarian carcinoma 2; differentially-expressed protein 2; disabled homolog 2; disabled homolog 2, mitogen-responsive phosphoprotein; DOC2; DOC-2; adaptor molecule disabled-2; Disabled homolog 2; DAB2, clathrin adaptor protein; anti-DAB2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-DAB2 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 300 and 350
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human Dab2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-Dab2 antibody AAA212438 (lot AAA212438-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human Dab2 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-Dab2 antibody AAA212438 (lot AAA212438-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human Dab2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Dab2 antibody AAA212438 (lot AAA212438-1) used for IP at 6 ug/mg lysate. Dab2 was also immunoprecipitated by rabbit anti-Dab2 antibody BL14430. For blotting immunoprecipitated Dab2, AAA212438 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human Dab2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Dab2 antibody AAA212438 (lot AAA212438-1) used for IP at 6 ug/mg lysate. Dab2 was also immunoprecipitated by rabbit anti-Dab2 antibody BL14430. For blotting immunoprecipitated Dab2, AAA212438 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Related Product Information for anti-DAB2 antibody
Disabled homolog 2 (Dab2) is an adapter protein that functions as a clathrin-associated sorting protein (CLASP) required for clathrin-mediated endocytosis of selected cargo proteins. Dab2 can bind and assemble clathrin, and binds simultaneously to phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) and cargos containing non-phosphorylated NPXY internalization motifs, such as the LDL receptor, to recruit them to clathrin-coated pits [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P98082].
Product Categories/Family for anti-DAB2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
80,228 Da
NCBI Official Full Name
disabled homolog 2 isoform 1
NCBI Official Synonym Full Names
Dab, mitogen-responsive phosphoprotein, homolog 2 (Drosophila)
NCBI Official Symbol
DAB2
NCBI Official Synonym Symbols
DOC2; DOC-2
NCBI Protein Information
disabled homolog 2; differentially-expressed protein 2; disabled homolog 2, mitogen-responsive phosphoprotein
UniProt Protein Name
Disabled homolog 2
UniProt Gene Name
DAB2
UniProt Synonym Gene Names
DOC2
UniProt Entry Name
DAB2_HUMAN
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Product Notes
The DAB2 dab2 (Catalog #AAA212438) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Dab2 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Dab2 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the DAB2 dab2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Dab2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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