WB (Western Blot)
(Detection of human DACH1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HEK293T (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), Jurkat (J; 50 ug) and HeLa (H; 50 ug) cells. Antibodies: Affinity purified rabbit anti-DACH1 antibody AAA212223 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. DACH1 was also immunoprecipitated by rabbit anti-DACH1 antibody which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).)
Rabbit anti-Human DACH1 Polyclonal Antibody | anti-DACH1 antibody
Rabbit anti-DACH1 Antibody, Affinity Purified
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
DACH1, Antibody; Rabbit anti-DACH1 Antibody, Affinity Purified; Dach1; dachshund homolog 1; dac homolog; DACH; Dachshund homolog 1; dachshund family transcription factor 1; anti-DACH1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-DACH1 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Mouse
Immunogen
Between 708 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human DACH1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HEK293T (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), Jurkat (J; 50 ug) and HeLa (H; 50 ug) cells. Antibodies: Affinity purified rabbit anti-DACH1 antibody AAA212223 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. DACH1 was also immunoprecipitated by rabbit anti-DACH1 antibody which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).)
WB (Western Blot)
(Detection of human DACH1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HEK293T (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), Jurkat (J; 50 ug) and HeLa (H; 50 ug) cells. Antibodies: Affinity purified rabbit anti-DACH1 antibody AAA212223 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. DACH1 was also immunoprecipitated by rabbit anti-DACH1 antibody which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).)
IHC (Immunohistochemistry)
(Detection of human DACH1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-DACH1 (Cat. No. AAA212223 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).)
IHC (Immunohistochemistry)
(Detection of human DACH1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-DACH1 (Cat. No. AAA212223 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).)
Related Product Information for anti-DACH1 antibody
Dachshund homolog 1 (Dach1) is a transcription factor that is involved in regulation of organogenesis. Dach1 seems to be a regulator of SIX1, SIX6 and probably SIX5. Corepression of precursor cell proliferation in myoblasts by SIX1 is switched to coactivation through recruitment of EYA3 to the SIX1-DACH1 complex. Transcriptional activation seems also to involve association of CREBBP. Dach1 seems to act as a corepressor of SIX6 in regulating proliferation by directly repressing cyclin-dependent kinase inhibitors, including the p27Kip1 promoter (by similarity) [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9UI36].
Product Categories/Family for anti-DACH1 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The DACH1 dach1 (Catalog #AAA212223) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-DACH1 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DACH1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the DACH1 dach1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DACH1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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