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IHC (Immunohistochemistry)
(DAG1 Antibody (C-term) (AAA285635) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DAG1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Rabbit DAG1 Polyclonal Antibody | anti-DAG1 antibody
DAG1 Antibody (C-term)
Gene Names
DAG1; A3a; DAG; AGRNR; 156DAG; MDDGC7; MDDGC9
Reactivity
Human, mouse (Predicted Reactivity: Pig, Rabbit)
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
DAG1, Antibody; DAG1 Antibody (C-term); Dystroglycan; Dystrophin-associated glycoprotein 1; Alpha-dystroglycan; Alpha-DG; Beta-dystroglycan; Beta-DG; DAG1; anti-DAG1 antibody
Host
Rabbit
Reactivity
Human, mouse (Predicted Reactivity: Pig, Rabbit)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This DAG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 718-747 amino acids from the C-terminal region of human DAG1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Positions
718-747
Sequence Length
895
Applicable Applications for anti-DAG1 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Function
The dystroglycan complex is involved in a number of processes including laminin and basement membrane assembly, sarcolemmal stability, cell survival, peripheral nerve myelination, nodal structure, cell migration, and epithelial polarization. [Beta-dystroglycan]: Transmembrane protein that plays important roles in connecting the extracellular matrix to the cytoskeleton. Acts as a cell adhesion receptor in both muscle and non- muscle tissues. Receptor for both DMD and UTRN and, through these interactions, scaffolds axin to the cytoskeleton. Also functions in cell adhesion-mediated signaling and implicated in cell polarity.
Cellular Location
[Alpha-dystroglycan]: Secreted, extracellular space
Tissue Location
Expressed in a variety of fetal and adult tissues. In epidermal tissue, located to the basement membrane. Also expressed in keratinocytes and fibroblasts.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistochemistry)
(DAG1 Antibody (C-term) (AAA285635) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DAG1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohistochemistry)
(DAG1 Antibody (C-term) (AAA285635) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DAG1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(All lanes : Anti-DAG1 Antibody (C-term) at 1:1000-1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 97 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-DAG1 Antibody (C-term) at 1:1000-1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 97 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-DAG1 Antibody (C-term) at 1:2000 dilution Lane 1: SH-SY5Y whole cell lysate Lane 2: NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 97 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-DAG1 Antibody (C-term) at 1:2000 dilution Lane 1: SH-SY5Y whole cell lysate Lane 2: NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 97 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-DAG1 Antibody (C-term) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 97 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-DAG1 Antibody (C-term) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 97 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-DAG1 Antibody (C-term) at 1:1000 dilution + NIH/3T3 whole cell lysate Lysates/proteins at 20ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 97 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-DAG1 Antibody (C-term) at 1:1000 dilution + NIH/3T3 whole cell lysate Lysates/proteins at 20ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 97 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-DAG1 antibody
Dystroglycan is a laminin binding component of the
dystrophin-glycoprotein complex which provides a linkage between
the subsarcolemmal cytoskeleton and the extracellular matrix.
Dystroglycan 1 is a candidate gene for the site of the mutation in
autosomal recessive muscular dystrophies. The dramatic reduction of
dystroglycan 1 in Duchenne muscular dystrophy leads to a loss of
linkage between the sarcolemma and extracellular matrix, rendering
muscle fibers more susceptible to necrosis. Dystroglycan also
functions as dual receptor for agrin and laminin-2 in the Schwann
cell membrane. The muscle and nonmuscle isoforms of dystroglycan
differ by carbohydrate moieties but not protein sequence.
Alternative splicing results in multiple transcript variants all
encoding the same protein.
dystrophin-glycoprotein complex which provides a linkage between
the subsarcolemmal cytoskeleton and the extracellular matrix.
Dystroglycan 1 is a candidate gene for the site of the mutation in
autosomal recessive muscular dystrophies. The dramatic reduction of
dystroglycan 1 in Duchenne muscular dystrophy leads to a loss of
linkage between the sarcolemma and extracellular matrix, rendering
muscle fibers more susceptible to necrosis. Dystroglycan also
functions as dual receptor for agrin and laminin-2 in the Schwann
cell membrane. The muscle and nonmuscle isoforms of dystroglycan
differ by carbohydrate moieties but not protein sequence.
Alternative splicing results in multiple transcript variants all
encoding the same protein.
Product Categories/Family for anti-DAG1 antibody
References
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Nilsson, J., et al. Glycobiology 20(9):1160-1169(2010)
Lara-Chacon, B., et al. J. Cell. Biochem. 110(3):706-717(2010)
Sgambato, A., et al. Pathology 42(3):248-254(2010)
Masaki, T., et al. J. Biomed. Biotechnol. 2010, 740403 (2010) :
Nilsson, J., et al. Glycobiology 20(9):1160-1169(2010)
Lara-Chacon, B., et al. J. Cell. Biochem. 110(3):706-717(2010)
Sgambato, A., et al. Pathology 42(3):248-254(2010)
Masaki, T., et al. J. Biomed. Biotechnol. 2010, 740403 (2010) :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
dystroglycan preproprotein
NCBI Official Synonym Full Names
dystroglycan 1 (dystrophin-associated glycoprotein 1)
NCBI Official Symbol
DAG1
NCBI Official Synonym Symbols
A3a; DAG; AGRNR; 156DAG; MDDGC7; MDDGC9
NCBI Protein Information
dystroglycan
UniProt Protein Name
Dystroglycan
UniProt Gene Name
DAG1
UniProt Synonym Gene Names
Alpha-DG; Beta-DG
UniProt Entry Name
DAG1_HUMAN
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Product Notes
The DAG1 dag1 (Catalog #AAA285635) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 718-747. The DAG1 Antibody (C-term) reacts with Human, mouse (Predicted Reactivity: Pig, Rabbit) and may cross-react with other species as described in the data sheet. AAA Biotech's DAG1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the DAG1 dag1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DAG1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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