Rabbit anti-Human, Mouse DAPK3 Polyclonal Antibody | anti-DAPK3 antibody

DAPK3 Antibody

Cat. Num. AAA232452

• Gene Names: DAPK3; DLK; ZIP; ZIPK
• Reactivity: Human, Mouse
• Applications: Immunofluorescence, Western Blot, ELISA
• Purity: >95%, Protein G purified
• Synonyms: DAPK3, Antibody; DAPK3 Antibody; Death-associated protein kinase 3; DAP-like kinase; Dlk; MYPT1 kinase; Zipper-interacting protein kinase; ZIP-kinase; DAPK3; ZIPK; anti-DAPK3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 454

• Applicable Applications for anti-DAPK3 antibody: IF (Immunofluorescence), WB (Western Blot), ELISA
• Immunogen: Recombinant Human Death-associated protein kinase 3 protein (283-401AA)
• Preservative: 0.03% Proclin 300
• Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Conjugation: Non-conjugated
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-8161 / sc-8162
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

IF (Immunofluorescence)

(Immunofluorescent analysis of Hela cells using AAA232452 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

WB (Western Blot)

(Western BlotPositive WB detected in:HepG2 whole cell lysate,Mouse brain tissueAll lanes: DAPK3 antibody at 3.4ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 53,38 kDaObserved band size: 53 kDa)

Related Product Information for anti-DAPK3 antibody

Serine/threonine kinase which is involved in the regulation of apoptosis, autophagy, transcription, translation and actin cytoskeleton reorganization. Involved in the regulation of smooth muscle contraction. Regulates both type I (caspase-dependent) apoptotic and type II (caspase-independent) autophagic cell deaths signal, depending on the cellular setting. Involved in regulation of starvation-induced autophagy. Regulates myosin phosphorylation in both smooth muscle and non-muscle cells. In smooth muscle, regulates myosin either directly by phosphorylating MYL12B and MYL9 or through inhibition of smooth muscle myosin phosphatase (SMPP1M) via phosphorylation of PPP1R12A; the inhibition of SMPP1M functions to enhance muscle responsiveness to Ca(2+) and promote a contractile state. Phosphorylates MYL12B in non-muscle cells leading to reorganization of actin cytoskeleton. Isoform 2 can phosphorylate myosin, PPP1R12A and MYL12B. Overexpression leads to condensation of actin stress fibers into thick bundles. Involved in actin filament focal adhesion dynamics. The function in both reorganization of actin cytoskeleton and focal adhesion dissolution is modulated by RhoD. Positively regulates canonical Wnt/beta-catenin signaling through interaction with NLK and TCF7L2. Phosphorylates RPL13A on 'Ser-77' upon interferon-gamma activation which is causing RPL13A release from the ribosome, RPL13A association with the GAIT complex and its subsequent involvement in transcript-selective translation inhibition. Enhances transcription from AR-responsive promoters in a hormone- and kinase-dependent manner. Involved in regulation of cell cycle progression and cell proliferation. May be a tumor suppressor.

NCBI and Uniprot Product Information

• NCBI GI #: 4557511
• NCBI GeneID: 1613
• NCBI Accession #: NP_001339.1
• NCBI GenBank Nucleotide #: NM_001348.2
• UniProt Accession #: O43293; Q05JY4; A0AVN4; B3KQE2
• Molecular Weight: 37,048 Da
• NCBI Official Full Name: death-associated protein kinase 3
• NCBI Official Synonym Full Names: death associated protein kinase 3
• NCBI Official Symbol: DAPK3
• NCBI Official Synonym Symbols: DLK; ZIP; ZIPK
• NCBI Protein Information: death-associated protein kinase 3
• UniProt Protein Name: Death-associated protein kinase 3
• UniProt Gene Name: DAPK3
• UniProt Synonym Gene Names: ZIPK; DAP kinase 3; Dlk; ZIP-kinase
• UniProt Entry Name: DAPK3_HUMAN