Rabbit DARPP-32 Polyclonal Antibody | anti-PPP1R1B antibody

Anti-DARPP-32

Cat. Num. AAA72784

• Gene Names: Ppp1r1b; Darpp32; Darpp-32
• Reactivity: Tested Reactivity: Mouse, Rat; Expected Reactivity: Bovine, Canine, Chicken, Human, Non-Human Primate, Xenopus
• Applications: Immunohistochemistry, Western Blot
• Purity: Affinity purified from Pooled Serum
• Synonyms: DARPP-32, Antibody; Anti-DARPP-32; anti-PPP1R1B antibody

• Host: Rabbit
• Reactivity: Tested Reactivity: Mouse, Rat; Expected Reactivity: Bovine, Canine, Chicken, Human, Non-Human Primate, Xenopus
• Clonality: Polyclonal
• Specificity: Specific for endogenous levels of the ~32 kDa DARPP-32 protein.
• Purity/Purification: Affinity purified from Pooled Serum
• Form/Format: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol.
• Sequence Length: 205

• Applicable Applications for anti-PPP1R1B antibody: IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen Information: Synthetic peptide corresponding to amino acid residues from the N-terminal region of rat DARPP-32, conjugated to keyhole limpet hemocyanin (KLH).
• Production Note: Prepared from pooled rabbit serum via chromatography on an affinity column prepared with the N-terminal peptide used as antigen.
• Preparation and Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol.

IHC (Immunohiostchemistry)

(Immunochemical staining of cultured CD1 mouse striatal cells showing specific labeling of DARPP-32(Cat. # 374-DARPP, green, 1:250) and Tuj1(red). Cells and photo courtesy of QBM Cell Science.)

WB (Western Blot)

(Western blot of rat hippocampal lysate showing specific immunolabeling of the ~32 kDa DARPP protein.)

Related Product Information for anti-PPP1R1B antibody

Description: Affinity purified rabbit polyclonal antibody
Target Description: DARPP-32 is a dopamine (DA) and cAMP-regulated ~32 kDa phosphoprotein that is associated with dopaminoceptive neurons (Fienberg et al., 1998). The protein inhibits protein phosphatase I when it is phosphorylated on Thr-34. In contrast, when DARPP-32 is phosphorylated on Thr-75 the protein acts as an inhibitor of PKA (Bibb et al., 1999). Phosphorylation of DARPP-32 is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac® (Maldve et al., 2002; Lindskog et al., 2002; Svenningsson et al., 2002).

References

• Bibb JA, Snyder GL, Nishi A, Yan Z, Meijer L, Fienberg AA, Tsai LH, Kwon YT, Girault JA, Czernik AJ, Huganir RL, Hemmings HC, Jr., Nairn AC, Greengard P (1999) Phosphorylation of DARPP-32 by cdk5 modulates dopamine signalling in neurons. Nature (London) 402:669-671.
• Fienberg, A.A., Hiroi, N., Mermelstein, P.G., Song, W., Snyder, G.L., Nishi, A., Cheramy, A. O'Callaghan, J.P., Miller, D.B., Cole, D.G., Corbett, R., Haile, C.N., Cooper, D,C., Onn, S.P., Grace, A.A., Ouimet, C.C., White, F.G., Hyman, S.E., Surmeier, D.G., Girault, J., Nestler, E.J. and Greengard, P. (1998) DARPP-32: regulator of the efficacy of dopaminergic neurotransmission. Science 281:838-842.
• Lindskog M, Svenningsson P, Pozzi L, Kim Y, Fienberg AA, Bibb JA, Fredholm BB, Nairn AC, Greengard P, Fisone G (2002) Involvement of DARPP-32 phosphorylation in the stimulant action of caffeine. Nature (London) 418:774- 778.
• Maldve RE, Zhang TA, Ferrani-Kile K, Schreiber SS, Lippmann MJ, Snyder GL, Feinberg AA, Leslie SW, Gonzales RA, Morrisett RA (2002) DARPP-32 and the regulation of the ethanol sensitivity of NMDA receptors in the nucleus accumbens. Nature Neurosci 5:641-648.
• Svenningsson P, Tzavara ET, Witkin JM, Fienberg AA, Nomikos GG, Greengard P (2002) Involvement of striatal and extrastriatal DARPP-32 in biochemical and behavioral effects of fluoxetine (Prozac). Proc Natl Acad Sci USA 99:3182-3187.

NCBI and Uniprot Product Information

• NCBI GI #: 61889054
• NCBI GeneID: 360616
• NCBI Accession #: NP_612530.1
• NCBI GenBank Nucleotide #: NM_138521.1
• UniProt Accession #: Q6J4I0
• Molecular Weight: 32kDa
• NCBI Official Full Name: protein phosphatase 1 regulatory subunit 1B
• NCBI Official Synonym Full Names: protein phosphatase 1, regulatory (inhibitor) subunit 1B
• NCBI Official Symbol: Ppp1r1b
• NCBI Official Synonym Symbols: Darpp32; Darpp-32
• NCBI Protein Information: protein phosphatase 1 regulatory subunit 1B; neuronal phosphoprotein DARPP-32; dopamine- and cAMP-regulated neuronal phosphoprotein; dopamine- and cAMP-regulated phosphoprotein DARPP-32
• UniProt Protein Name: Protein phosphatase 1 regulatory subunit 1B
• UniProt Gene Name: Ppp1r1b
• UniProt Entry Name: PPR1B_RAT