IP (Immunoprecipitation)
(TNFRSF10C was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate4 uL anti-TNFRSF10C rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-TNFRSF10C rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 65 kDaObserved band size :65 kDa)
Rabbit anti-Human DcR1/TRAILR3 Polyclonal Antibody | anti-TNFRSF10C antibody
Anti-DcR1/TRAILR3 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
DcR1/TRAILR3, Antibody; Anti-DcR1/TRAILR3 Antibody, Rabbit Polyclonal; DcR1/TRAILR3 Antibody, Rabbit PAb, Antigen Affinity Purified; Anti-CD263 Antibody; Anti-DCR1 Antibody; Anti-DCR1-TNFR Antibody; Anti-LIT Antibody; Anti-TRAIL-R3 Antibody; Anti-TRAILR3 Antibody; Anti-TRID Antibody; tumor necrosis factor receptor superfamily member 10c; anti-TNFRSF10C antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human DcR1/TRAILR3
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid. PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-TNFRSF10C antibody
IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot)
Immunogen
E Coli-derived Human DcR1/TRAILR3 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human DcR1/TRAILR3 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
IP (Immunoprecipitation)
(TNFRSF10C was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate4 uL anti-TNFRSF10C rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-TNFRSF10C rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 65 kDaObserved band size :65 kDa)
IP (Immunoprecipitation)
(TNFRSF10C was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate4 uL anti-TNFRSF10C rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-TNFRSF10C rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 65 kDaObserved band size :65 kDa)
IF (Immunofluorescence)
(Immunofluorescence staining of TRAILR3 in A549 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human TRAILR3 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor-488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)
IF (Immunofluorescence)
(Immunofluorescence staining of TRAILR3 in A549 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human TRAILR3 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor-488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)
WB (Western Blot)
(Anti-DcR1/TRAILR3 rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:65 kDaObserved band size:70 kDa)
WB (Western Blot)
(Anti-DcR1/TRAILR3 rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:65 kDaObserved band size:70 kDa)
Related Product Information for anti-TNFRSF10C antibody
Background: TNFRSF10C CNV in patients with CRC is associated with distant metastatic disease. A high frequency of CGI methylation in the TNFRSF10C promoter results in inactivation of the gene and enhancement of tumor growth in most PC cell lines (except CFPAC-1). Inactivation of TNFRSF10C by CpG island (CGI) hypermethylation can play an important role in PC progression and be potentially useful as a test marker and a new therapeutic approach for PC.
Product Categories/Family for anti-TNFRSF10C antibody
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Product Notes
The TNFRSF10C (Catalog #AAA257751) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-DcR1/TRAILR3 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DcR1/TRAILR3 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the TNFRSF10C for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DcR1/TRAILR3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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