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WB (Western Blot)
(Anti-TNFRSF10D rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Membrane LysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:42 kDaObserved band size:45 kDa)
Rabbit anti-Human DcR2/TRAIL R4 Polyclonal Antibody | anti-TNFRSF10D antibody
Anti-DcR2/TRAIL R4 Antibody, Rabbit Polyclonal
Gene Names
TNFRSF10D; DCR2; CD264; TRUNDD; TRAILR4; TRAIL-R4
Reactivity
Human
Applications
Immunoprecipitation, Immunohistochemistry, ELISA, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
DcR2/TRAIL R4, Antibody; Anti-DcR2/TRAIL R4 Antibody, Rabbit Polyclonal; TRAIL R4/CD264/TNFRSF10D Antibody, Rabbit PAb, Antigen Affinity Purified; tumor necrosis factor receptor superfamily, member 10d, decoy with truncated death domain; Anti-CD264 Antibody; Anti-DCR2 Antibody; Anti-TRAIL-R4 Antibody; Anti-TRAILR4 Antibody; Anti-TRUNDD Antibody; anti-TNFRSF10D antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human TRAIL R4/CD264/TNFRSF10D
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-TNFRSF10D antibody
IP (Immunoprecipitation), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Immunogen
Recombinant Human TRAIL R4/CD264/TNFRSF10D protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human TRAIL R4/CD264/TNFRSF10D (rh TRAIL R4/CD264/TNFRSF10D). TRAIL R4/CD264/TNFRSF10D specific IgG was purified by Human TRAIL R4/CD264/TNFRSF10D affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
WB (Western Blot)
(Anti-TNFRSF10D rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Membrane LysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:42 kDaObserved band size:45 kDa)
WB (Western Blot)
(Anti-TNFRSF10D rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Membrane LysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:42 kDaObserved band size:45 kDa)
IP (Immunoprecipitation)
(TNFRSF10D was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate0.5 uL anti-TNFRSF10D rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-TNFRSF10D rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 42 kDaObserved band size: 42 kDa)
IP (Immunoprecipitation)
(TNFRSF10D was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate0.5 uL anti-TNFRSF10D rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-TNFRSF10D rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 42 kDaObserved band size: 42 kDa)
IHC (Immunohistochemisry)
(Immunochemical staining of human TNFRSF10D in human kidney with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemisry)
(Immunochemical staining of human TNFRSF10D in human kidney with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohiostchemistry)
(Immunochemical staining of human TNFRSF10D in human liver with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohiostchemistry)
(Immunochemical staining of human TNFRSF10D in human liver with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry)
(Immunochemical staining of human TNFRSF10D in human testis with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry)
(Immunochemical staining of human TNFRSF10D in human testis with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.)
Related Product Information for anti-TNFRSF10D antibody
Tumor necrosis factor receptor superfamily member 10D (TNFRSF10D), also known as TNF-related apoptosis-inducing ligand receptor 4 (TRAIL R4), CD264, and Decoy receptor 2, is a member of the TNF-receptor superfamily. This receptor contains an extracellular TRAIL-binding domain, a transmembrane domain, and a truncated cytoplamic death domain. This receptor does not induce apoptosis, and has been shown to play an inhibitory role in TRAIL-induced cell apoptosis. TRAIL R4/CD264/TNFRSF10D is widely expressed, in particular in fetal kidney, lung and liver, and in adult testis and liver. TRAIL R4/CD264/TNFRSF10D is also expressed in peripheral blood leukocytes, colon and small intestine, ovary, prostate, thymus, spleen, pancreas, kidney, lung, placenta and heart. The signaling capacity of TRAIL R4 is similar to that of TRAIL R1 and TRAIL R2 with respect to NF-kappaB activation, but differs in its inability to induce apoptosis. TRAIL R4 retains a C-terminal element containing one third of a consensus death domain motif. Transient overexpression of TRAIL R4 in cells normally sensitive to TRAIL-mediated killing confers complete protection, suggesting that one function of TRAIL R4 may be inhibition of TRAIL cytotoxicity.
Product Categories/Family for anti-TNFRSF10D antibody
References
Degli-Esposti MA, et al. (1997) The novel receptor TRAIL-R4 induces NF-kappaB and protects against TRAIL-mediated apoptosis, yet retains an incomplete death domain. Immunity. 7(6): 813-20.Meng RD, et al. (2000) The TRAIL decoy receptor TRUNDD (DcR2, TRAIL-R4) is induced by adenovirus-p53 overexpression and can delay TRAIL-, p53-, and KILLER/DR5-dependent colon cancer apoptosis. Mol Ther. 1(2): 130-44.Bouralexis S, et al. (2003) Progressive resistance of BTK-143 osteosarcoma cells to Apo2L/TRAIL-induced apoptosis is mediated by acquisition of DcR2/TRAIL-R4 expression: resensitisation with chemotherapy. Br J Cancer. 89(1): 206-14.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
41,823 Da
NCBI Official Full Name
tumor necrosis factor receptor superfamily member 10D
NCBI Official Synonym Full Names
tumor necrosis factor receptor superfamily, member 10d, decoy with truncated death domain
NCBI Official Symbol
TNFRSF10D
NCBI Official Synonym Symbols
DCR2; CD264; TRUNDD; TRAILR4; TRAIL-R4
NCBI Protein Information
tumor necrosis factor receptor superfamily member 10D; TRAIL receptor 4; decoy receptor 2; TNF receptor-related receptor for TRAIL; TRAIL receptor with a truncated death domain; TNF-related apoptosis-inducing ligand receptor 4
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 10D
UniProt Gene Name
TNFRSF10D
UniProt Synonym Gene Names
DCR2; TRAILR4; TRUNDD; DcR2; TRAIL receptor 4; TRAIL-R4
UniProt Entry Name
TR10D_HUMAN
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Product Notes
The TNFRSF10D tnfrsf10d (Catalog #AAA254909) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-DcR2/TRAIL R4 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DcR2/TRAIL R4 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the TNFRSF10D tnfrsf10d for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DcR2/TRAIL R4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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