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IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded human lung cancer using DDB1 Antibody at dilution of 1:100 (40x lens).)
Rabbit DDB1 Polyclonal Antibody | anti-DDB1 antibody
DDB1 Polyclonal Antibody
Gene Names
DDB1; XPE; DDBA; XAP1; XPCE; XPE-BF; UV-DDB1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purification
Synonyms
DDB1; Polyclonal Antibody; DDB1 Polyclonal Antibody; XPE; DDBA; XAP1; XPCE; XPE-BF; UV-DDB1; anti-DDB1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Concentration
1mg/ml (varies by lot)
Sequence Length
1140
Applicable Applications for anti-DDB1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500 - 1:1000
IHC: 1:50 - 1:100
IHC: 1:50 - 1:100
Species
Human
Route
Synthetic Peptide
Immunogen
A synthetic peptide of human DDB1
Calculated Molecular Weight
127kDa
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded human lung cancer using DDB1 Antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded human lung cancer using DDB1 Antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded rat brain using DDB1 Antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded rat brain using DDB1 Antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded rat testis using DDB1 Antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded rat testis using DDB1 Antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded human gastric cancer using DDB1 Antibody at dilution of 1:200 (40x lens).)
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded human gastric cancer using DDB1 Antibody at dilution of 1:200 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human kidney cancer using DDB1 Antibody at dilution of 1:200 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human kidney cancer using DDB1 Antibody at dilution of 1:200 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human liver injury using DDB1 Antibody at dilution of 1:200 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human liver injury using DDB1 Antibody at dilution of 1:200 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse testis using DDB1 Antibody at dilution of 1:200 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse testis using DDB1 Antibody at dilution of 1:200 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human uterine cancer using DDB1 Antibody at dilution of 1:200 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human uterine cancer using DDB1 Antibody at dilution of 1:200 (40x lens).)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using DDB1 Antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using DDB1 Antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)
Related Product Information for anti-DDB1 antibody
The protein encoded by this gene is the large subunit (p127) of the heterodimeric DNA damage-binding (DDB) complex while another protein (p48) forms the small subunit. This protein complex functions in nucleotide-excision repair and binds to DNA following UV damage. Defective activity of this complex causes the repair defect in patients with xeroderma pigmentosum complementation group E (XPE) - an autosomal recessive disorder characterized by photosensitivity and early onset of carcinomas. However, it remains for mutation analysis to demonstrate whether the defect in XPE patients is in this gene or the gene encoding the small subunit. In addition, Best vitelliform mascular dystrophy is mapped to the same region as this gene on 11q, but no sequence alternations of this gene are demonstrated in Best disease patients. The protein encoded by this gene also functions as an adaptor molecule for the cullin 4 (CUL4) ubiquitin E3 ligase complex by facilitating the binding of substrates to this complex and the ubiquitination of proteins.
Product Categories/Family for anti-DDB1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
1140
NCBI Official Full Name
DNA damage-binding protein 1
NCBI Official Synonym Full Names
damage-specific DNA binding protein 1, 127kDa
NCBI Official Symbol
DDB1
NCBI Official Synonym Symbols
XPE; DDBA; XAP1; XPCE; XPE-BF; UV-DDB1
NCBI Protein Information
DNA damage-binding protein 1; XAP-1; UV-DDB 1; DDB p127 subunit; XPE-binding factor; HBV X-associated protein 1; DNA damage-binding protein a; UV-damaged DNA-binding factor; UV-damaged DNA-binding protein 1; xeroderma pigmentosum group E-complementing protein
UniProt Protein Name
DNA damage-binding protein 1
UniProt Gene Name
DDB1
UniProt Synonym Gene Names
XAP1; DDBa; XAP-1; UV-DDB 1; XPE-BF; XPCe
UniProt Entry Name
DDB1_HUMAN
Similar Products
Product Notes
The DDB1 ddb1 (Catalog #AAA10733) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DDB1 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's DDB1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500 - 1:1000 IHC: 1:50 - 1:100. Researchers should empirically determine the suitability of the DDB1 ddb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DDB1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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