Rabbit DDIT4 Polyclonal Antibody | anti-DDIT4 antibody

DDIT4 Antibody (C-term)

Cat. Num. AAA287533

• Gene Names: DDIT4; Dig2; REDD1; REDD-1
• Reactivity: Human (Predicted Reactivity: Bovine, Mouse, Rat)
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: DDIT4, Antibody; DDIT4 Antibody (C-term); DNA damage-inducible transcript 4 protein; HIF-1 responsive protein RTP801; Protein regulated in development and DNA damage response 1; REDD-1; DDIT4; REDD1; RTP801; anti-DDIT4 antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Bovine, Mouse, Rat)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: This DDIT4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 198-227 amino acids from the C-terminal region of human DDIT4.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Sequence Positions: 198-227
• Sequence Length: 232

• Applicable Applications for anti-DDIT4 antibody: FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
• Function: Regulates cell growth, proliferation and survival via inhibition of the activity the mammalian target of rapamycin complex 1 (Mtorc1). Inhibition of Mtorc1 is mediated by a pathway that involves DDIT4/ REDD1. AKT1, the TSCI- TSC2 complex and the GTPase RHEB. Plays an important role in responses to cellular energy level and cellular stress, including responses to hypoxia and DNA damage. Regulates p53/TP53- mediated apoptosis in response to DNA damage via its effect on Mtorci1 inhibition in fibroblasts and thymocites, but not in hepatocytes (By similarity). Required for Mtorci-1- mediated defense against viral protein synthesis and virus replication ( By on MTorc1 activity . Required for normal neuron migration during embryonic brain development. Plays a role in neuronal cell death.
• Cellular location: Mitochondrion, Cytoplasm, Cytosol.
• Tissue location: Broadly expressed with lowest level in brain, skeletal muscle and intestine. Up- regulated in substantia nigra neurons from Parkinson disease patients (at protein level).
• Preparation and Storage: Maintain refrigerated at 2-8 degree for short tem.. For long term storage store at -20 degree in small aliquots to prevent freeze-thaw cycles.

FCM/FACS (Flow Cytometry)

(DDIT4 Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohiostchemistry)

(DDIT4 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DDIT4 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot)

(Western blot analysis of DDIT4 (arrow) using rabbit polyclonal DDIT4 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DDIT4 gene.)

Related Product Information for anti-DDIT4 antibody

REDD1 is a novel transcriptional target of p53 induced following DNA damage. During embryogenesis, REDD1 expression mirrors the tissue-specific pattern of the p53 family member p63, and TP63 null embryos show virtually no expression of REDD1, which is restored in mouse embryo fibroblasts following p63 expression. In differentiating primary keratinocytes, TP63 and REDD1 expression are coordinately downregulated, and ectopic expression of either gene inhibits in vitro differentiation. REDD1 appears to function in the regulation of reactive oxygen species (ROS); TP63 null fibroblasts have decreased ROS levels and reduced sensitivity to oxidative stress, which are both increased following ectopic expression of either TP63 or REDD1. Thus, REDD1 encodes a shared transcriptional target that implicates ROS in the p53-dependent DNA damage response and in p63-mediated regulation of epithelial differentiation.

Product Categories/Family for anti-DDIT4 antibody

Cancer; Cardiovascular; Metabolism

References

Ellisen L.W., Mol. Cell 10:995-1005(2002).
Shoshani T.,Mol. Cell. Biol. 22:2283-2293(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 9506687
• NCBI GeneID: 54541
• NCBI Accession #: NP_061931.1
• NCBI GenBank Nucleotide #: NM_019058.2
• UniProt Accession #: Q9NX09; Q9H0S3
• Molecular Weight: Calculated MW: 25371
• NCBI Official Full Name: DNA damage-inducible transcript 4 protein
• NCBI Official Synonym Full Names: DNA-damage-inducible transcript 4
• NCBI Official Symbol: DDIT4
• NCBI Official Synonym Symbols: Dig2; REDD1; REDD-1
• NCBI Protein Information: DNA damage-inducible transcript 4 protein
• UniProt Protein Name: DNA damage-inducible transcript 4 protein
• UniProt Gene Name: DDIT4
• UniProt Synonym Gene Names: REDD1; RTP801; REDD-1
• UniProt Entry Name: DDIT4_HUMAN