WB (Western Blot)
(Anti-DDR1 rabbit polyclonal antibody at 1:500 dilutionLane A: MCF7 Membrane LysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:101 kDaObserved band size:130 kDa)
Rabbit anti-Human DDR1 Polyclonal Antibody | anti-DDR1 antibody
Anti-DDR1 Antibody, Rabbit Polyclonal
Gene Names
DDR1; CAK; DDR; NEP; HGK2; PTK3; RTK6; TRKE; CD167; EDDR1; MCK10; NTRK4; PTK3A; DDR1
Reactivity
Human
Applications
Immunoprecipitation, ELISA, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
DDR1, Antibody; Anti-DDR1 Antibody, Rabbit Polyclonal; DDR1 Kinase/MCK10/CD167 Antibody, Rabbit PAb, Antigen Affinity Purified; discoidin domain receptor tyrosine kinase 1; Anti-CAK Antibody; Anti-CD167 Antibody; Anti-DDR Antibody; Anti-EDDR1 Antibody; Anti-HGK2 Antibody; Anti-MCK10 Antibody; Anti-NEP Antibody; Anti-NTRK4 Antibody; Anti-PTK3 Antibody; Anti-PTK3A Antibody; Anti-RTK6 Antibody; Anti-TRKE Antibody; anti-DDR1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human DDR1/CD167a
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-DDR1 antibody
IP (Immunoprecipitation), ELISA, WB (Western Blot)
Immunogen
Recombinant Human DDR1 protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human DDR1/CD167a (rh DDR1). DDR1 specific IgG was purified by human DDR1 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
WB (Western Blot)
(Anti-DDR1 rabbit polyclonal antibody at 1:500 dilutionLane A: MCF7 Membrane LysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:101 kDaObserved band size:130 kDa)
WB (Western Blot)
(Anti-DDR1 rabbit polyclonal antibody at 1:500 dilutionLane A: MCF7 Membrane LysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:101 kDaObserved band size:130 kDa)
IP (Immunoprecipitation)
(DDR1 was immunoprecipitated using:Lane A:0.5 mg SW480 Whole Cell LysateLane B:0.5 mg MCF-7 Whole Cell Lysate0.5 uL anti-DDR1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-DDR1 rabbit polyclonal antibody,at 1:1000 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 101 kDaObserved band size: 130 kDa)
IP (Immunoprecipitation)
(DDR1 was immunoprecipitated using:Lane A:0.5 mg SW480 Whole Cell LysateLane B:0.5 mg MCF-7 Whole Cell Lysate0.5 uL anti-DDR1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-DDR1 rabbit polyclonal antibody,at 1:1000 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 101 kDaObserved band size: 130 kDa)
Related Product Information for anti-DDR1 antibody
Discoidin domain receptor family, member 1 (DDR1), also known as or CD167a (cluster of differentiation 167a), and Mammary carcinoma kinase 10 (MCK10), belongs to a subfamily of tyrosine kinase receptors with an extracellular domain homologous to Dictyostellium discoideum protein discoidin 1. Receptor tyrosine kinases play a key role in the communication of cells with their microenvironment. These kinases are involved in the regulation of cell growth, differentiation and metabolism. Expression of DDR1/MCK10/CD167 is restricted to epithelial cells, particularly in the kidney, lung, gastrointestinal tract, and brain. In addition, it has been shown to be significantly overexpressed in several human tumors. DDR1/MCK10/CD167 plays an important role in regulating attachment to collagen, chemotaxis, proliferation, and MMP production in smooth muscle cells. DDR1 functions in a feedforward loop to increase p53 levels and at least some of its effectors. Inhibition of DDR1 function resulted in strikingly increased apoptosis of wild-type p53-containing cells in response to genotoxic stress through a caspase-dependent pathway.
Product Categories/Family for anti-DDR1 antibody
References
Hou G, et al. (2001) The discoidin domain receptor tyrosine kinase DDR1 in arterial wound repair. J Clin Invest. 107(6): 727-35.Ongusaha PP, et al. (2003) p53 induction and activation of DDR1 kinase counteract p53-mediated apoptosis and influence p53 regulation through a positive feedback loop. EMBO J. 22(6): 1289-301.Jonsson M, et al. (2001) Repression of Wnt-5a impairs DDR1 phosphorylation and modifies adhesion and migration of mammary cells. J Cell Sci. 114(11): 2043-53.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
epithelial discoidin domain-containing receptor 1 isoform DDR1a [Homo sapiens]
NCBI Official Synonym Full Names
discoidin domain receptor tyrosine kinase 1
NCBI Official Symbol
DDR1
NCBI Official Synonym Symbols
CAK; DDR; NEP; HGK2; PTK3; RTK6; TRKE; CD167; EDDR1; MCK10; NTRK4; PTK3A; DDR1
UniProt Protein Name
Epithelial discoidin domain-containing receptor 1
UniProt Gene Name
DDR1
UniProt Synonym Gene Names
CAK; EDDR1; NEP; NTRK4; PTK3A; RTK6; TRKE; Epithelial discoidin domain receptor 1; MCK-10
UniProt Entry Name
DDR1_HUMAN
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Product Notes
The DDR1 ddr1 (Catalog #AAA255117) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-DDR1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DDR1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the DDR1 ddr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DDR1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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