WB (Western Blot)
(Detection of human DDX21 by western blot. Samples: Whole cell lysate from HeLa cells. Antibodies: Affinity purified rabbit anti-DDX21 antibody AAA211062 (lot AAA211062-1) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with exposure time of 10 seconds.)
Rabbit anti-Human DDX21 Polyclonal Antibody | anti-DDX21 antibody
Rabbit anti-DDX21 Antibody, Affinity Purified
Gene Names
DDX21; GUA; GURDB; RH-II/GU; RH-II/GuA; DKFZp686F21172
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
DDX21, Antibody; Rabbit anti-DDX21 Antibody, Affinity Purified; nucleolar RNA helicase 2; RNA helicase II/Gu alpha; RH-II/GuA; RH-II/GU; RH II/Gu; nucleolar RNA helicase II; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 21; nucleolar RNA helicase Gu; gu-alpha; GUA; DEAD-box helicase 21; Gu protein; DEAD box protein 21; DEAD (Asp-Glu-Ala-Asp) box polypeptide 21; DEAD (Asp-Glu-Ala-Asp) box helicase 21; GURDB; Nucleolar RNA helicase 2; DExD-box helicase 21; anti-DDX21 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-DDX21 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 75 and 125
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human DDX21 by western blot. Samples: Whole cell lysate from HeLa cells. Antibodies: Affinity purified rabbit anti-DDX21 antibody AAA211062 (lot AAA211062-1) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with exposure time of 10 seconds.)
WB (Western Blot)
(Detection of human DDX21 by western blot. Samples: Whole cell lysate from HeLa cells. Antibodies: Affinity purified rabbit anti-DDX21 antibody AAA211062 (lot AAA211062-1) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human DDX21 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-DDX21 antibody AAA211062 (lot AAA211062-1) used for IP at 3 ug/mg lysate. DDX21 was also immunoprecipitated using rabbit anti-DDX21 antibodies and at 3 ug/mg lysate. For blotting immunoprecipitated DDX21, AAA211062 was used at 1 ug/ml. Detection: Chemiluminescence with exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human DDX21 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-DDX21 antibody AAA211062 (lot AAA211062-1) used for IP at 3 ug/mg lysate. DDX21 was also immunoprecipitated using rabbit anti-DDX21 antibodies and at 3 ug/mg lysate. For blotting immunoprecipitated DDX21, AAA211062 was used at 1 ug/ml. Detection: Chemiluminescence with exposure time of 3 seconds.)
Related Product Information for anti-DDX21 antibody
DDX21 is a member of the DEAD box family of proteins that possesses several conserved motifs which include the highly conserved DEAD (Asp-Glu-Ala-Asp) amino acid sequence motif. The major activity of DEAD box proteins is to function as ATP-dependent RNA helicases. As helicases, DEAD proteins play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. Some members of the DEAD box proteins also exhibit functions involved in transcriptional regulation. DDX21 has been shown to be required for the processing of 20S rRNA to 18S and has also been shown to be important for c-Jun transcriptional activation.
Product Categories/Family for anti-DDX21 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
87,344 Da
NCBI Official Full Name
nucleolar RNA helicase 2
NCBI Official Synonym Full Names
DEAD (Asp-Glu-Ala-Asp) box polypeptide 21
NCBI Official Symbol
DDX21
NCBI Official Synonym Symbols
GUA; GURDB; RH-II/GU; RH-II/GuA; DKFZp686F21172
NCBI Protein Information
nucleolar RNA helicase 2; RH II/Gu; gu-alpha; Gu protein; OTTHUMP00000019713; DEAD box protein 21; RNA helicase II/Gu alpha; nucleolar RNA helicase Gu; nucleolar RNA helicase II; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 21
UniProt Protein Name
Nucleolar RNA helicase 2
UniProt Gene Name
DDX21
UniProt Entry Name
DDX21_HUMAN
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Product Notes
The DDX21 ddx21 (Catalog #AAA211062) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-DDX21 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DDX21 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the DDX21 ddx21 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DDX21, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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