Rabbit anti-Human, Mouse DDX3 Polyclonal Antibody | anti-DDX3X antibody

DDX3 Antibody

Cat. Num. AAA39369

• Gene Names: DDX3X; DBX; DDX3; HLP2; DDX14; CAP-Rf
• Reactivity: Human, Mouse
• Applications: Immunofluorescence, Immunocytochemistry, Western Blot, ELISA
• Purity: DDX3 Antibody is affinity chromatography purified via peptide column.
• Synonyms: DDX3, Antibody; DDX3 Antibody; DDX3; DBX; HLP2; DDX14; ATP-dependent RNA helicase DDX3X; DEAD box protein 3; X-chromosomal; DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked; anti-DDX3X antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: DDX3 antibody will detect both DDX3 and DBY.
• Purity/Purification: DDX3 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 662

• Applicable Applications for anti-DDX3X antibody: IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot), ELISA
• Conjugate: Unconjugated
• Immunogen: DDX3 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human DDX3.
• Buffer: DDX3 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: DDX3 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IF (Immunofluorescence)

(Immunofluorescence of DDX3 in HepG2 cells with DDX3 antibody at 20 μg/mL.)

ICC (Immunocytochemistry)

(Immunocytochemistry of DDX3 in HepG2 cells with DDX3 antibody at 10 μg/mL.)

WB (Western Blot)

(Western blot analysis of DDX3 in HepG2 cell lysate with DDX3 antibody at (A) 0.5 and (B) 1 μg/mL.)

Related Product Information for anti-DDX3X antibody

DDX3 Antibody: DDX3 contains all of the motifs of the DEAD-box family of RNA helicases, including the Asp-Glu-Ala-Asp sequence that gives the protein family its name and distinguishes it from other RNA helicases. DDX3 is localized to the X chromosome and has a highly conserved functional homolog (DBY) on the Y chromosome. DDX3 is thought to be involved in RNA splicing, RNA transport, and translation initiation. It has also been shown to be involved in cell growth control and is deregulated in hepatitis virus-associated hepatocellular carcinoma. Recent experiments suppressing DDX3 expression blocked HIV-1 RNA export from the nucleus, suggesting that DDX3 functions as a shuttling protein that transports the HIV-1 protein Rev and its cofactor CRM1 from the nucleus to the cytoplasm.

NCBI and Uniprot Product Information

• NCBI GI #: 3523150
• NCBI GeneID: 1654
• UniProt Accession #: O00571; O15536; A8K538; B4E3E8
• Molecular Weight: 71,355 Da
• NCBI Official Full Name: DEAD box RNA helicase DDX3
• NCBI Official Synonym Full Names: DEAD (Asp-Glu-Ala-Asp) box helicase 3, X-linked
• NCBI Official Symbol: DDX3X
• NCBI Official Synonym Symbols: DBX; DDX3; HLP2; DDX14; CAP-Rf
• NCBI Protein Information: ATP-dependent RNA helicase DDX3X; DEAD/H box-3; DEAD box, X isoform; helicase-like protein 2; DEAD box protein 3, X-chromosomal; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3; DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked
• UniProt Protein Name: ATP-dependent RNA helicase DDX3X
• UniProt Gene Name: DDX3X
• UniProt Synonym Gene Names: DBX; DDX3; HLP2
• UniProt Entry Name: DDX3X_HUMAN