IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with DDX3 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Rabbit DDX3 Polyclonal Antibody | anti-DDX3X antibody
DDX3 Antibody (C-term)
Gene Names
DDX3X; DBX; DDX3; HLP2; DDX14; CAP-Rf
Reactivity
Human (Predicted Reactivity: Mouse, Xenopus)
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
DDX3, Antibody; DDX3 Antibody (C-term); ATP-dependent RNA helicase DDX3X; DEAD box protein 3; X-chromosomal; DEAD box; X isoform; Helicase-like protein 2; HLP2; DDX3X; DBX; DDX3; anti-DDX3X antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse, Xenopus)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This DDX3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 456-485 amino acids from the C-terminal region of human DDX3.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
456-485
Sequence Length
646
Applicable Applications for anti-DDX3X antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with DDX3 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with DDX3 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of DDX3 Antibody (C-term) in Hela cell line lysates (35ug/lane). DDX3 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of DDX3 Antibody (C-term) in Hela cell line lysates (35ug/lane). DDX3 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of DDX3 (arrow) using rabbit polyclonal DDX3 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the DDX3 gene (Lane 2) (Origene Technologies).)
WB (Western Blot)
(Western blot analysis of DDX3 (arrow) using rabbit polyclonal DDX3 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the DDX3 gene (Lane 2) (Origene Technologies).)
Related Product Information for anti-DDX3X antibody
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX3 is a DEAD box protein, which interacts specifically with hepatitis C virus core protein resulting a change in intracellular location. The DDX3 gene has a homolog located in the nonrecombining region of the Y chromosome. The protein sequence is 91% identical between this gene and the Y-linked homolog.
Product Categories/Family for anti-DDX3X antibody
References
Ariumi,Y., J. Virol. 81 (24), 13922-13926 (2007)
Hogbom,M., J. Mol. Biol. 372 (1), 150-159 (2007)
Franca,R., Proteins 67 (4), 1128-1137 (2007)
DDX3, Cancer Res. 66 (13), 6579-6588 (2006)
Hogbom,M., J. Mol. Biol. 372 (1), 150-159 (2007)
Franca,R., Proteins 67 (4), 1128-1137 (2007)
DDX3, Cancer Res. 66 (13), 6579-6588 (2006)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
73243
NCBI Official Full Name
ATP-dependent RNA helicase DDX3X isoform 3
NCBI Official Synonym Full Names
DEAD (Asp-Glu-Ala-Asp) box helicase 3, X-linked
NCBI Official Symbol
DDX3X
NCBI Official Synonym Symbols
DBX; DDX3; HLP2; DDX14; CAP-Rf
NCBI Protein Information
ATP-dependent RNA helicase DDX3X
UniProt Protein Name
ATP-dependent RNA helicase DDX3X
UniProt Gene Name
DDX3X
UniProt Synonym Gene Names
DBX; DDX3; HLP2
UniProt Entry Name
DDX3X_HUMAN
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Product Notes
The DDX3X ddx3x (Catalog #AAA289370) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 456-485. The DDX3 Antibody (C-term) reacts with Human (Predicted Reactivity: Mouse, Xenopus) and may cross-react with other species as described in the data sheet. AAA Biotech's DDX3 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the DDX3X ddx3x for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DDX3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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