IHC (Immunohiostchemistry)
(AAA327869 at 1/100 staining Mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit anti-Human, Mouse DDX42 Polyclonal Antibody | anti-DDX42 antibody
DDX42 Antibody
Gene Names
DDX42; RHELP; RNAHP; SF3B8; DDX42P; SF3b125
Reactivity
Human, Mouse
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
DDX42, Antibody; DDX42 Antibody; ATP-dependent RNA helicase DDX42; DEAD (Asp-Glu-Ala-Asp) box polypeptide 42; DEAD box polypeptide 42 protein; DEAD box protein 42; EC 3.6.1.-; FLJ43179; RHELP; RNA helicase-like protein; RNA helicase-related protein; RNAHP; SF3b DEAD-box protein; SF3b125; SF3b125 DEAD-box protein; Splicing factor 3B-associated 125 kDa protein; anti-DDX42 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
DDX42 antibody detects endogenous levels of total DDX42
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
938
Applicable Applications for anti-DDX42 antibody
ELISA, IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human DDX42
Subcellular Location
Nucleus
Tissue Specificity
Expressed in several cell lines (at protein level). Expressed in liver, lung, tonsil, thymus, muscle and pancreatic islets.
Predicted Cross Reactivity
Pig, Bovine, Horse, Rabbit, Dog
Similarity
Pig (100%), Bovine (100%), Horse (100%), Rabbit (100%), Dog (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IHC (Immunohiostchemistry)
(AAA327869 at 1/100 staining Mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohiostchemistry)
(AAA327869 at 1/100 staining Mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot)
(Western blot analysis of DDX42 using HuvEc whole cell lysates)
WB (Western Blot)
(Western blot analysis of DDX42 using HuvEc whole cell lysates)
Related Product Information for anti-DDX42 antibody
Function: ATP-dependent RNA helicase. Binds to partially double-stranded RNAs (dsRNAs) in order to unwind RNA secondary structures. Unwinding is promoted in the presence of single-strand binding proteins. Mediates also RNA duplex formation thereby displacing the single-strand RNA binding protein. ATP and ADP modulate its activity: ATP binding and hydrolysis by DDX42 triggers RNA strand separation, whereas the ADP-bound form of the protein triggers annealing of complementary RNA strands. Involved in the survival of cells by interacting with TP53BP2 and thereby counteracting the apoptosis-stimulating activity of TP53BP2. Relocalizes TP53BP2 to the cytoplasm.
Subunit Structure: Component of splicing factor SF3B complex which is composed of at least eight subunits; SF3B1, SF3B2, SF3B3, SF3B4, SF3B5, SF3B6, PHF5A/SF3B14B, and DDX42/SF3B125. Interacts (via the C-terminus) with TP53BP2; the interaction is not inhibitied by TP53BP2 ubiquitination and is independent of p53/TP53.
Similarity: Belongs to the DEAD box helicase family. DDX42 subfamily.
Subunit Structure: Component of splicing factor SF3B complex which is composed of at least eight subunits; SF3B1, SF3B2, SF3B3, SF3B4, SF3B5, SF3B6, PHF5A/SF3B14B, and DDX42/SF3B125. Interacts (via the C-terminus) with TP53BP2; the interaction is not inhibitied by TP53BP2 ubiquitination and is independent of p53/TP53.
Similarity: Belongs to the DEAD box helicase family. DDX42 subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 103 kDa
Predicted: 103 kDa
Predicted: 103 kDa
NCBI Official Full Name
ATP-dependent RNA helicase DDX42
NCBI Official Synonym Full Names
DEAD-box helicase 42
NCBI Official Symbol
DDX42
NCBI Official Synonym Symbols
RHELP; RNAHP; SF3B8; DDX42P; SF3b125
NCBI Protein Information
ATP-dependent RNA helicase DDX42
UniProt Protein Name
ATP-dependent RNA helicase DDX42
UniProt Gene Name
DDX42
UniProt Synonym Gene Names
RHELP; RNAHP; SF3b125
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Product Notes
The DDX42 ddx42 (Catalog #AAA327869) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DDX42 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's DDX42 can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the DDX42 ddx42 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DDX42, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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