Rabbit anti-Human, Mouse DDX5 Polyclonal Antibody | anti-DDX5 antibody

Rabbit anti-DDX5 Antibody, Affinity Purified

Cat. Num. AAA210999

• Gene Names: DDX5; p68; HLR1; G17P1; HUMP68
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Antigen Affinity Purified
• Synonyms: DDX5, Antibody; Rabbit anti-DDX5 Antibody, Affinity Purified; probable ATP-dependent RNA helicase DDX5; RNA helicase p68; p68; HUMP68; HLR1; G17P1; DEAD box-5; DEAD box protein 5; DEAD (Asp-Glu-Ala-Asp) box polypeptide 5; DEAD (Asp-Glu-Ala-Asp) box helicase 5; ATP-dependent RNA helicase DDX5; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5 (RNA helicase, 68kD); Probable ATP-dependent RNA helicase DDX5; DEAD-box helicase 5; anti-DDX5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Purity/Purification: Antigen Affinity Purified
• Form/Format: Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
• Concentration: 200ug/ml (varies by lot)

• Applicable Applications for anti-DDX5 antibody: IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
• Predicted Reactivity: Orangutan, Chimpanzee
• Immunogen: Between 564 and C-terminus
• Conjugation: Unconjugated
• Preparation and Storage: Store at 2-8 degree C; Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of mouse DDX5 by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DDX5 antibody AAA210999 (lot AAA210999-3) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)

WB (Western Blot)

(Detection of human DDX5 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DDX5 antibody AAA210999 (lot AAA210999-3) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)

IP (Immunoprecipitation)

(Detection of human DDX5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DDX5 antibody AAA210999 (lot AAA210999-3) used for IP at 6 ug per reaction. DDX5 was also immunoprecipitated by a previous lot of this antibody (lot AAA210999-2) and rabbit anti-DDX5 antibody For blotting immunoprecipitated DDX5, AAA210999 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)

IHC (Immunohiostchemistry)

(Detection of mouse DDX5 by immunohistochemistry. Sample: FFPE section of mouse skin. Antibody: Affinity purified rabbit anti-DDX5 Cat. No. AAA210999 lot 3 used at a dilution of 1:1000 (0.2ug/ml). Detection: DAB)

IHC (Immunohistochemistry)

(Detection of human DDX5 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-DDX5 Cat. No. AAA210999 lot 3 used at a dilution of 1:1000 (0.2ug/ml). Detection: DAB)

Related Product Information for anti-DDX5 antibody

DDX5 is a member of the DEAD box family of proteins that possesses several conserved motifs which include the highly conserved DEAD (Asp-Glu-Ala-Asp) amino acid sequence motif. The major activity of DEAD box proteins is to function as ATP-dependent RNA helicases. As helicases, DEAD proteins play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. Some members of the DEAD box proteins also exhibit functions involved in transcriptional regulation. In addition to a role involved in RNA splicing, DDX5 has been observed to interact with and act as a transcriptional coactivator for estrogen receptor alpha (ERalpha). DDX5 has also been found to interact with CBP/300, Smad3, and p53 to modulate transcription. DDX5 is closely related to and forms heterodimers with DDX17.

Product Categories/Family for anti-DDX5 antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 4758138
• NCBI GeneID: 1655
• NCBI Accession #: NP_004387.1
• NCBI GenBank Nucleotide #: NM_004396.3
• UniProt Accession #: P17844; O75681; Q53Y61; B5BU21; D3DU32
• Molecular Weight: 69,148 Da
• NCBI Official Full Name: probable ATP-dependent RNA helicase DDX5
• NCBI Official Synonym Full Names: DEAD (Asp-Glu-Ala-Asp) box helicase 5
• NCBI Official Symbol: DDX5
• NCBI Official Synonym Symbols: p68; HLR1; G17P1; HUMP68
• NCBI Protein Information: probable ATP-dependent RNA helicase DDX5; DEAD box-5; RNA helicase p68; DEAD box protein 5; DEAD (Asp-Glu-Ala-Asp) box polypeptide 5; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5 (RNA helicase, 68kD)
• UniProt Protein Name: Probable ATP-dependent RNA helicase DDX5
• UniProt Gene Name: DDX5
• UniProt Synonym Gene Names: G17P1; HELR; HLR1
• UniProt Entry Name: DDX5_HUMAN