Rabbit DDX60 Polyclonal Antibody | anti-DDX60 antibody

DDX60 Antibody

Cat. Num. AAA40515

• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Western Blot, ELISA
• Purity: DDX60 Antibody is affinity chromatography purified via peptide column.
• Synonyms: DDX60, Antibody; DDX60 Antibody; DDX60; Probable ATP-dependent RNA helicase DDX60; DEAD box protein 60; DEAD (Asp-Glu-Ala-Asp) box polypeptide 60; anti-DDX60 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: At least three isoforms of DDX60 are known to exist; this antibody will detect all three isoforms.
• Purity/Purification: DDX60 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 1712

• Applicable Applications for anti-DDX60 antibody: IF (Immunofluorescence), WB (Western Blot), ELISA
• Conjugate: Unconjugated
• Immunogen: DDX60 antibody was raised against a 17 amino acid synthetic peptide near the center of human DDX60.
• Buffer: DDX60 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: DDX60 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IF (Immunofluorescence)

(Immunofluorescence of DDX60 in A20 cells with DDX60 antibody at 20 μg/mL.)

WB (Western Blot)

(Western blot analysis of DDX60 in A20 cell lysate with DDX60 antibody at (A) 1 and (B) 2 μg/mL.)

Related Product Information for anti-DDX60 antibody

DDX60 Antibody: DDX60 is a DEXD/H Box RNA helicase and the human homolog of the S. cerevisiae Ski2, a cofactor of RNA exosome. Expression of DDX60 increases after viral infection and binds to endogenous RIG-I, a cytoplasmic viral RNA sensor, as well as other RIG-I-like receptors such as MDA5 and LGP2. These proteins activate TBK1 and IKK-e, signaling IRF3 and NF-kB-responsive genes in the innate immune response pathway. DDX60 is required for the RIG-I- or MDA5-dependent response to viral infection, but is dispensable for TLR3-mediated signaling.

NCBI and Uniprot Product Information

• NCBI GI #: 222831595
• NCBI GeneID: 55601
• NCBI Accession #: NP_060101
• NCBI GenBank Nucleotide #: NM_017631.5
• UniProt Accession #: Q8IY21; Q6PK35; Q9NVE3
• Molecular Weight: 188 kDa
• NCBI Official Full Name: probable ATP-dependent RNA helicase DDX60
• NCBI Official Synonym Full Names: DEAD (Asp-Glu-Ala-Asp) box polypeptide 60
• NCBI Official Symbol: DDX60
• NCBI Protein Information: probable ATP-dependent RNA helicase DDX60; DEAD box protein 60
• UniProt Protein Name: Probable ATP-dependent RNA helicase DDX60
• UniProt Gene Name: DDX60
• UniProt Entry Name: DDX60_HUMAN