Rabbit DEN1A Polyclonal Antibody | anti-DENND1A antibody

DEN1A Antibody (C-term)

Cat. Num. AAA286888

• Gene Names: DENND1A; FAM31A; KIAA1608
• Reactivity: Human (Predicted Reactivity: Mouse)
• Applications: Western Blot, Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA
• Purity: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Synonyms: DEN1A, Antibody; DEN1A Antibody (C-term); DENN domain-containing protein 1A; Connecdenn 1; Connecdenn; Protein FAM31A; DENND1A; FAM31A; KIAA1608; anti-DENND1A antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Mouse)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This DEN1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 576-605 amino acids from the C-terminal region of human DEN1A.
• Purity/Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Positions: 576-605

• Applicable Applications for anti-DENND1A antibody: WB (Western Blot), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

FCM/FACS (Flow Cytometry)

(DEN1A Antibody (C-term) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohistochemisry)

(DEN1A Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human cervix carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DEN1A Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot)

(Anti-DEN1A Antibody (C-term) at 1:500 dilution + human liver lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 111 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot)

(Western blot analysis of DEN1A Antibody (C-term) in K562 cell line lysates (35ug/lane). DEN1A (arrow) was detected using the purified Pab.)

Product Categories/Family for anti-DENND1A antibody

Neuroscience; Signal Transduction

References

Allaire, P.D., et al. J. Neurosci. 26(51):13202-13212(2006)
Olsen, J.V., et al. Cell 127(3):635-648(2006)

NCBI and Uniprot Product Information

• NCBI GI #: 55749779
• NCBI GeneID: 57706
• NCBI Accession #: NP_065997.1; NP_079096.2
• NCBI GenBank Nucleotide #: NM_020946.1
• UniProt Accession #: Q8TEH3; Q05C88; Q5VWF0; Q6PJZ5; Q8IVD6; Q9H796; A8MZA3; B1AM80; B7Z3C8; B7Z669; D3PFD3
• Molecular Weight: 110577
• NCBI Official Full Name: DENN domain-containing protein 1A isoform 1
• NCBI Official Synonym Full Names: DENN/MADD domain containing 1A
• NCBI Official Symbol: DENND1A
• NCBI Official Synonym Symbols: FAM31A; KIAA1608
• NCBI Protein Information: DENN domain-containing protein 1A
• UniProt Protein Name: DENN domain-containing protein 1A
• UniProt Gene Name: DENND1A
• UniProt Synonym Gene Names: FAM31A; KIAA1608; Connecdenn
• UniProt Entry Name: DEN1A_HUMAN