FCM/FACS (Flow Cytometry)
(DEN5A Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit DEN5A Polyclonal Antibody | anti-DENND5A antibody
DEN5A Antibody (Center)
Gene Names
DENND5A; RAB6IP1
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
DEN5A, Antibody; DEN5A Antibody (Center); DENN domain-containing protein 5A; Rab6-interacting protein 1; Rab6IP1; DENND5A; KIAA1091; RAB6IP1; anti-DENND5A antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This DEN5A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 695-724 amino acids from the Central region of human DEN5A.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
695-724
Sequence Length
1241
Applicable Applications for anti-DENND5A antibody
FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(DEN5A Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(DEN5A Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(DEN5A Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the DEN5A antibody detected the DEN5A protein (arrow).)
WB (Western Blot)
(DEN5A Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the DEN5A antibody detected the DEN5A protein (arrow).)
Related Product Information for anti-DENND5A antibody
May be involved in Rab6-mediated GTPase signaling.
Product Categories/Family for anti-DENND5A antibody
References
Recacha, R., et al. Structure 17(1):21-30(2009)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Janoueix-Lerosey, I., et al. J. Biol. Chem. 270(24):14801-14808(1995)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Janoueix-Lerosey, I., et al. J. Biol. Chem. 270(24):14801-14808(1995)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
147096
NCBI Official Full Name
DENN domain-containing protein 5A isoform 2
NCBI Official Synonym Full Names
DENN/MADD domain containing 5A
NCBI Official Symbol
DENND5A
NCBI Official Synonym Symbols
RAB6IP1
NCBI Protein Information
DENN domain-containing protein 5A
UniProt Protein Name
DENN domain-containing protein 5A
UniProt Gene Name
DENND5A
UniProt Synonym Gene Names
KIAA1091; RAB6IP1; Rab6IP1
UniProt Entry Name
DEN5A_HUMAN
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Product Notes
The DENND5A dennd5a (Catalog #AAA288287) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 695-724. The DEN5A Antibody (Center) reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's DEN5A can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the DENND5A dennd5a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DEN5A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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