IHC (Immunohiostchemistry)
(Immunochemical staining of human DENND2D in human stomach with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)
Rabbit anti-Human DENND2D Polyclonal Antibody | anti-DENND2D antibody
Anti-DENND2D Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
DENND2D, Antibody; Anti-DENND2D Antibody, Rabbit Polyclonal; DENND2D Antibody, Rabbit PAb, Antigen Affinity Purified; DENN domain containing 2D; anti-DENND2D antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human DENND2D
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-DENND2D antibody
IHC (Immunohistochemistry)
Immunogen
E Coli-derived Human DENND2D fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human DENND2D fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
IHC (Immunohiostchemistry)
(Immunochemical staining of human DENND2D in human stomach with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohiostchemistry)
(Immunochemical staining of human DENND2D in human stomach with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry)
(Immunochemical staining of human DENND2D in human urinary bladder with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry)
(Immunochemical staining of human DENND2D in human urinary bladder with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)
Related Product Information for anti-DENND2D antibody
The DENN/MADD domain-containing (DENND) proteins regulate Rab GTPases and represent a newly recognized class of membrane trafficking proteins. DENND proteins directly interact with Rab35 and function as guanine nucleotide exchange factors (GEFs) for this GTPase. DENN/MADD domain containing 2D (DENND2D), a member of the DENND2 family, is located on chromosome 1p13.3 and encodes a 53-kDa protein that is a candidate tumor suppressor gene. Silencing via promoter hypermethylation regulates DENND2D in hepatocellular carcinoma (HCC), esophageal squamous cell carcinoma (ESCC) and gastric cancer (GC). DENND2D also reportedly suppresses the proliferation and tumorigenicity of NSCLC cells. DENND2D is a candidate tumor suppressor gene that is inactivated by promoter hypermethylation in patients with hepatocellular carcinoma (HCC) and may serve as a novel biomarker of early recurrence of HCC.
References
Zhang T, et al. (2016) Downregulation of mir-522 suppresses proliferation and metastasis of non-small cell lung cancer cells by directly targeting denn/madd domain containing 2d. Sci Rep 6 19346.Kanda M, et al. (2014) Downregulation of dennd2d by promoter hypermethylation is associated with early recurrence of hepatocellular carcinoma. Int J Oncol 44 (1): 44-52.Hibino S, et al. (2014) Reduced expression of dennd2d through promoter hypermethylation is an adverse prognostic factor in squamous cell carcinoma of the esophagus. Oncol Rep 31 (2): 693-700.
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Product Notes
The DENND2D (Catalog #AAA256291) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-DENND2D Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DENND2D can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the DENND2D for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DENND2D, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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