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product-image-AAA212450_WB10.jpg WB (Western Blot) (Detection of human DENTT by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat cells. Antibodies: Affinity purified rabbit anti-DENTT antibody AAA212450 (lot AAA212450-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)

Rabbit anti-Human DENTT Polyclonal Antibody | anti-DENTT antibody

Rabbit anti-DENTT Antibody, Affinity Purified

Gene Names
TSPYL2; CDA1; CTCL; NP79; TSPX; CINAP; DENTT; SE204; HRIHFB2216
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Antigen Affinity Purified
Synonyms
DENTT, Antibody; Rabbit anti-DENTT Antibody, Affinity Purified; differentially-expressed nucleolar TGF-beta1 target protein; HRIHFB2216; NP79; nuclear protein of 79 kDa; SE204; testis-specific protein Y encoded-like 2; testis-specific Y-encoded-like protein 2; TSPX; differentially expressed nucleolar TGF-beta1 target; TSPY-like protein 2; cell division autoantigen 1; CDA1; DENTT; CASK-interacting nucleosome assembly protein; CINAP; CTCL; CTCL tumor antigen se20-4; CTCL-associated antigen se20-4; cutaneous T-cell lymphoma-associated antigen se20-4; cutaneous T-cell lymphoma-associated tumor antigen se20-4; Testis-specific Y-encoded-like protein 2; TSPY like 2; anti-DENTT antibody
Ordering
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-DENTT antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
Between 643 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of human DENTT by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat cells. Antibodies: Affinity purified rabbit anti-DENTT antibody AAA212450 (lot AAA212450-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)

product-image-AAA212450_WB10.jpg WB (Western Blot) (Detection of human DENTT by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat cells. Antibodies: Affinity purified rabbit anti-DENTT antibody AAA212450 (lot AAA212450-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)

IP (Immunoprecipitation)

(Detection of human DENTT by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-DENTT antibody AAA212450 (lot AAA212450-1) used for IP at 6 ug/mg lysate. DENTT was also immunoprecipitated by rabbit anti-DENTT antibody For blotting immunoprecipitated DENTT, AAA212450 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)

product-image-AAA212450_IP11.jpg IP (Immunoprecipitation) (Detection of human DENTT by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-DENTT antibody AAA212450 (lot AAA212450-1) used for IP at 6 ug/mg lysate. DENTT was also immunoprecipitated by rabbit anti-DENTT antibody For blotting immunoprecipitated DENTT, AAA212450 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)

IHC (Immunohiostchemistry)

(Detection of human DENTT by immunohistochemistry. Samples: FFPE serial sections of human lung cancer. Antibody: Affinity purified rabbit anti-DENTT (Cat. No. Lot1, left image and Cat. No. AAA212450 Lot1, right image) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)

product-image-AAA212450_IHC13.jpg IHC (Immunohiostchemistry) (Detection of human DENTT by immunohistochemistry. Samples: FFPE serial sections of human lung cancer. Antibody: Affinity purified rabbit anti-DENTT (Cat. No. Lot1, left image and Cat. No. AAA212450 Lot1, right image) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)

IHC (Immunohistochemistry)

(Detection of human DENTT by immunohistochemistry. Samples: FFPE serial sections of human testis. Antibody: Affinity purified rabbit anti-DENTT (Cat. No. Lot1, left image and Cat. No. AAA212450 Lot1, right image) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)

product-image-AAA212450_IHC15.jpg IHC (Immunohistochemistry) (Detection of human DENTT by immunohistochemistry. Samples: FFPE serial sections of human testis. Antibody: Affinity purified rabbit anti-DENTT (Cat. No. Lot1, left image and Cat. No. AAA212450 Lot1, right image) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-DENTT antibody
Differentially-expressed nucleolar TGF-beta1 target protein (DENTT) is part of the CASK/TRB1/TSPYL2 transcriptional complex which modulates gene expression in response to neuronal synaptic activity, probably by facilitating nucleosome assembly. DENTT may inhibit cell proliferation by inducing p53-dependent CDKN1A expression [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9H2G4].
Product Categories/Family for anti-DENTT antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
79,435 Da
NCBI Official Full Name
testis-specific Y-encoded-like protein 2
NCBI Official Synonym Full Names
TSPY-like 2
NCBI Official Symbol
TSPYL2
NCBI Official Synonym Symbols
CDA1; CTCL; NP79; TSPX; CINAP; DENTT; SE204; HRIHFB2216
NCBI Protein Information
testis-specific Y-encoded-like protein 2; TSPY-like protein 2; CTCL tumor antigen se20-4; nuclear protein of 79 kDa; cell division autoantigen 1; CTCL-associated antigen se20-4; testis-specific protein Y encoded-like 2; CASK-interacting nucleosome assembl
UniProt Protein Name
Testis-specific Y-encoded-like protein 2
UniProt Gene Name
TSPYL2
UniProt Synonym Gene Names
CDA1; DENTT; TSPX; TSPY-like protein 2; CTCL-associated antigen se20-4; NP79
UniProt Entry Name
TSYL2_HUMAN

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Product Notes

The DENTT tspyl2 (Catalog #AAA212450) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-DENTT Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DENTT can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the DENTT tspyl2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DENTT, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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