WB (Western Blot)
(Western blot analysis of extracts from Hela, using Phospho-DEPDC5 (S1002) Antibody.)
Rabbit DEPDC5 Polyclonal Antibody | anti-DEPDC5 antibody
Phospho-DEPDC5 (Ser1002) Antibody
Gene Names
DEPDC5; DEP.5; FFEVF
Applications
Western Blot
Purity
The Ab is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Synonyms
DEPDC5, Antibody; Phospho-DEPDC5 (Ser1002) Antibody; DEP domain containing 5; DEP domain-containing protein 5; DEP.5; DEPD5_HUMAN; DEPDC5; FFEVF; KIAA0645; anti-DEPDC5 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
Rabbit IgG
Purity/Purification
The Ab is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Form/Format
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
~1mg/ml (varies by lot)
Applicable Applications for anti-DEPDC5 antibody
WB (Western Blot)
Immunogen
A synthesized peptide derived from human DEPDC5 around the phosphorylation site of S1002.
Important
For western blot, incubate membrane with diluted primary Ab in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
Preparation and Storage
Store at -20 °C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of extracts from Hela, using Phospho-DEPDC5 (S1002) Antibody.)
WB (Western Blot)
(Western blot analysis of extracts from Hela, using Phospho-DEPDC5 (S1002) Antibody.)
WB (Western Blot)
(Western blot analysis of extracts from Hela cells, using Phospho-DEPDC5 (Ser1002) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from Hela cells, using Phospho-DEPDC5 (Ser1002) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)
Related Product Information for anti-DEPDC5 antibody
As a component of the GATOR1 complex functions as an inhibitor of the amino acid-sensing branch of the TORC1 pathway. The GATOR1 complex strongly increases GTP hydrolysis by RRAGA and RRAGB within RRAGC-containing heterodimers, thereby deactivating RRAGs, releasing mTORC1 from lysosomal surface and inhibiting mTORC1 signaling. The GATOR1 complex is negatively regulated by GATOR2 the other GATOR subcomplex in this amino acid-sensing branch of the TORC1 pathway.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
180 kDa
NCBI Official Full Name
DEP domain-containing protein 5
NCBI Official Synonym Full Names
DEP domain containing 5
NCBI Official Symbol
DEPDC5
NCBI Official Synonym Symbols
DEP.5; FFEVF
NCBI Protein Information
DEP domain-containing protein 5
UniProt Protein Name
DEP domain-containing protein 5
UniProt Gene Name
DEPDC5
UniProt Synonym Gene Names
KIAA0645
UniProt Entry Name
DEPD5_HUMAN
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Product Notes
The DEPDC5 depdc5 (Catalog #AAA329974) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's DEPDC5 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the DEPDC5 depdc5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DEPDC5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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